Effect of metabolosome encapsulation peptides on enzyme activity, coaggregation, incorporation, and bacterial microcompartment formation.
bacterial organelles
cargo
protein aggregation
synthetic biology
targeting
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
08
10
2019
revised:
17
01
2020
accepted:
21
01
2020
pubmed:
14
2
2020
medline:
30
4
2021
entrez:
14
2
2020
Statut:
ppublish
Résumé
Metabolosomes, catabolic bacterial microcompartments (BMCs), are proteinaceous organelles that are associated with the breakdown of metabolites such as propanediol and ethanolamine. They are composed of an outer multicomponent protein shell that encases a specific metabolic pathway. Protein cargo found within BMCs is directed by the presence of an encapsulation peptide that appears to trigger aggregation before the formation of the outer shell. We investigated the effect of three distinct encapsulation peptides on foreign cargo in a recombinant BMC system. Our data demonstrate that these peptides cause variations in enzyme activity and protein aggregation. We observed that the level of protein aggregation generally correlates with the size of metabolosomes, while in the absence of cargo BMCs self-assemble into smaller compartments. The results agree with a flexible model for BMC formation based around the ability of the BMC shell to associate with an aggregate formed due to the interaction of encapsulation peptides.
Identifiants
pubmed: 32053746
doi: 10.1002/mbo3.1010
pmc: PMC7221423
doi:
Substances chimiques
Bacterial Proteins
0
Peptides
0
Recombinant Proteins
0
Metallothionein
9038-94-2
Pyruvate Decarboxylase
EC 4.1.1.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L01386X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M002969/1
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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