Metabolic engineering of type II methanotroph, Methylosinus trichosporium OB3b, for production of 3-hydroxypropionic acid from methane via a malonyl-CoA reductase-dependent pathway.
3-hydroxypropionic acid
Acetyl-CoA carboxylase
Acetyl-CoA carboxylation bypass
Malonyl-CoA reductase
Methane
Methylosinus trichosporium OB3b
Journal
Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
01
10
2019
revised:
07
01
2020
accepted:
09
02
2020
pubmed:
18
2
2020
medline:
28
1
2021
entrez:
17
2
2020
Statut:
ppublish
Résumé
We engineered a type II methanotroph, Methylosinus trichosporium OB3b, for 3-hydroxypropionic acid (3HP) production by reconstructing malonyl-CoA pathway through heterologous expression of Chloroflexus aurantiacus malonyl-CoA reductase (MCR), a bifunctional enzyme. Two strategies were designed and implemented to increase the malonyl-CoA pool and thus, increase in 3HP production. First, we engineered the supply of malonyl-CoA precursors by overexpressing endogenous acetyl-CoA carboxylase (ACC), substantially enhancing the production of 3HP. Overexpression of biotin protein ligase (BPL) and malic enzyme (NADP
Identifiants
pubmed: 32061966
pii: S1096-7176(20)30035-5
doi: 10.1016/j.ymben.2020.02.002
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Lactic Acid
33X04XA5AT
hydracrylic acid
C4ZF6XLD2X
Oxidoreductases
EC 1.-
malonyl-Coa reductase
EC 1.-
Methane
OP0UW79H66
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
142-150Informations de copyright
Copyright © 2020 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.