Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.


Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 May 2020
Historique:
received: 02 12 2019
revised: 11 02 2020
accepted: 15 02 2020
pubmed: 20 2 2020
medline: 4 2 2021
entrez: 20 2 2020
Statut: ppublish

Résumé

Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds. Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity. In this study, fucoidan was extracted from E. maxima by the hot water extraction method and then characterised by colorimetric assays for sugar composition. The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate. The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa. Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature. Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract. Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC

Identifiants

pubmed: 32070744
pii: S0141-8130(19)39663-1
doi: 10.1016/j.ijbiomac.2020.02.161
pii:
doi:

Substances chimiques

Glycoside Hydrolase Inhibitors 0
Hypoglycemic Agents 0
Polysaccharides 0
Starch 9005-25-8
fucoidan 9072-19-9
alpha-Glucosidases EC 3.2.1.20

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

412-420

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Chantal Désirée Daub (CD)

Department of Biochemistry and Microbiology, Rhodes University, 6140 Grahamstown, South Africa.

Blessing Mabate (B)

Department of Biochemistry and Microbiology, Rhodes University, 6140 Grahamstown, South Africa.

Samkelo Malgas (S)

Department of Biochemistry and Microbiology, Rhodes University, 6140 Grahamstown, South Africa.

Brett Ivan Pletschke (BI)

Department of Biochemistry and Microbiology, Rhodes University, 6140 Grahamstown, South Africa. Electronic address: b.pletschke@ru.ac.za.

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Classifications MeSH