The Oviductal Extracellular Vesicles' RNA Cargo Regulates the Bovine Embryonic Transcriptome.
Animals
Cattle
Cluster Analysis
Embryo, Mammalian
/ cytology
Embryonic Development
/ genetics
Extracellular Vesicles
/ metabolism
Female
Freezing
Gene Expression Regulation, Developmental
MicroRNAs
/ metabolism
Oviducts
/ cytology
RNA
/ chemistry
RNA, Messenger
/ metabolism
Sequence Analysis, RNA
Transcriptome
EV RNA cargo
EV-derived mRNAs
EV-derived miRNAs
embryo
exosomes
extracellular vesicles
gene expression
oviduct
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
14 Feb 2020
14 Feb 2020
Historique:
received:
21
01
2020
revised:
11
02
2020
accepted:
12
02
2020
entrez:
21
2
2020
pubmed:
23
2
2020
medline:
21
11
2020
Statut:
epublish
Résumé
Oviductal extracellular vesicles (oEVs) are emerging as key players in the gamete/embryo-oviduct interactions that contribute to successful pregnancy. Various positive effects of oEVs on gametes and early embryos have been found in vitro. To determine whether these effects are associated with changes of embryonic gene expression, the transcriptomes of embryos supplemented with bovine fresh (FeEVs) or frozen (FoEVs) oEVs during in vitro culture compared to controls without oEVs were analyzed by low-input RNA sequencing. Analysis of RNA-seq data revealed 221 differentially expressed genes (DEGs) between FoEV treatment and control, 67 DEGs for FeEV and FoEV treatments, and minor differences between FeEV treatment and control (28 DEGs). An integrative analysis of mRNAs and miRNAs contained in oEVs obtained in a previous study with embryonic mRNA alterations pointed to direct effects of oEV cargo on embryos (1) by increasing the concentration of delivered transcripts; (2) by translating delivered mRNAs to proteins that regulate embryonic gene expression; and (3) by oEV-derived miRNAs which downregulate embryonic mRNAs or modify gene expression in other ways. Our study provided the first high-throughput analysis of the embryonic transcriptome regulated by oEVs, increasing our knowledge on the impact of oEVs on the embryo and revealing the oEV RNA components that potentially regulate embryonic development.
Identifiants
pubmed: 32075098
pii: ijms21041303
doi: 10.3390/ijms21041303
pmc: PMC7072903
pii:
doi:
Substances chimiques
MicroRNAs
0
RNA, Messenger
0
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Swiss National Science Foundation
ID : SNSF grant 31003A_173171
Pays : Switzerland
Organisme : French National Institute for Agricultural Research - INRA
ID : INRA-CI_PHASE
Organisme : European Union in the framework of H2020-SFS-2015-2
ID : Grant agreement n°677353-2 IMAGE
Organisme : EU COST-Action
ID : EPICONCEPT-FA1201
Organisme : EU COST-Action
ID : SALAAM- BM1308
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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