MCSeEd (Methylation Context Sensitive Enzyme ddRAD): A New Method to Analyze DNA Methylation.

DNA Methylation / genetics DNA, Plant / genetics Epigenesis, Genetic / genetics Genome, Plant / genetics Stress, Physiological / genetics Zea mays / genetics
DNA methylation Development MCSEeD Methylation context sensitive enzyme ddRAD Regulation gene expression Sequencing Zea mays

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 24 2 2020
pubmed: 24 2 2020
medline: 30 12 2020
Statut: ppublish

Résumé

Methylation context sensitive enzyme ddRAD (MCSeEd) is a NGS-based method for genome-wide investigations of DNA methylation at different contexts requiring only low to moderate sequencing depth. It is particularly useful for identifying methylation changes in experimental systems challenged by biotic or abiotic stresses or at different developmental stages.

Identifiants

DOI: 10.1007/978-1-0716-0179-2_4 PMID: 32088888
pubmed: 32088888
doi: 10.1007/978-1-0716-0179-2_4
doi:

Substances chimiques

DNA, Plant 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

47-64

Auteurs

Marco Di Marsico (M)

Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy.

Elisa Cerruti (E)

Department of Agricultural, Forest and Food Sciences, Plant Genetics and Breeding, University of Torino, Grugliasco, Italy.

Cinzia Comino (C)

Department of Agricultural, Forest and Food Sciences, Plant Genetics and Breeding, University of Torino, Grugliasco, Italy.

Andrea Porceddu (A)

Department of Agriculture, University of Sassari, Sassari, Italy.

Alberto Acquadro (A)

Department of Agricultural, Forest and Food Sciences, Plant Genetics and Breeding, University of Torino, Grugliasco, Italy.

Stefano Capomaccio (S)

Department of Veterinary Medicine, University of Perugia, Perugia, Italy.

Gianpiero Marconi (G)

Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy. gianpiero.marconi@unipg.it.

Emidio Albertini (E)

Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Méthylation de l'ADN › MCSeEd (Methylation Context Sensitive Enzyme...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN des plantes › MCSeEd (Methylation Context Sensitive Enzyme...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › MCSeEd (Methylation Context Sensitive Enzyme...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome végétal › MCSeEd (Methylation Context Sensitive Enzyme...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › MCSeEd (Methylation Context Sensitive Enzyme...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Zea mays › MCSeEd (Methylation Context Sensitive Enzyme...