NTF-RINT, a new method for the epidemiological surveillance of MDR Mycobacterium tuberculosis L2/Beijing strains.
Bacteriological Techniques
DNA Mutational Analysis
DNA Transposable Elements
DNA, Bacterial
/ genetics
Drug Resistance, Multiple, Bacterial
/ genetics
Genotype
High-Throughput Nucleotide Sequencing
Humans
Kazakhstan
/ epidemiology
Molecular Epidemiology
Mutation
Mycobacterium tuberculosis
/ genetics
New York City
/ epidemiology
Phenotype
Polymerase Chain Reaction
Population Surveillance
Predictive Value of Tests
Reproducibility of Results
Tuberculosis, Multidrug-Resistant
/ diagnosis
Virulence
Atypical Beijing
MDR-TB
Molecular epidemiology
Typical Beijing
Journal
Tuberculosis (Edinburgh, Scotland)
ISSN: 1873-281X
Titre abrégé: Tuberculosis (Edinb)
Pays: Scotland
ID NLM: 100971555
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
22
08
2019
revised:
29
11
2019
accepted:
08
12
2019
entrez:
25
2
2020
pubmed:
25
2
2020
medline:
4
9
2021
Statut:
ppublish
Résumé
The most widely discussed antibiotic-resistant tuberculosis strains ("W" and "B0/W148", "CAO") belong to L2/Beijing Lineage and are characterized by IS6110 insertion sequences at the NTF locus. We present a high-throughput, microbead-based method, called NTF-RINT for detection of IS in NTF and Rifampicin and Isoniazid Typing. This method provides tuberculosis diagnostic confirmation, screens for the so-called modern L2/Beijing sublineage and detects mutations involved in resistance to Rifampicin (RIF) and Isoniazid (INH).
Identifiants
pubmed: 32090855
pii: S1472-9792(19)30315-4
doi: 10.1016/j.tube.2019.101894
pii:
doi:
Substances chimiques
DNA Transposable Elements
0
DNA, Bacterial
0
Types de publication
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101894Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of competing interest Bernice Klotoe was partly founded by Beamedex®, a society that sells microbead-based kits. The society had however no part in the decision of what new tests could be set-up and this was entirely decided by public researchers Christophe Sola, Guislaine Refrégier, with the consent of Natalia Kurepina and Barry N. Kreiswirth. Guislaine Refrégier acknowledges one travel grant by Luminex® on another project regarding SNP detection. Luminex® is a manufacturer of microbead-based instruments and microbeads.