Fluorogenic glycopolymers available for determining the affinity of lectins by intermolecular FRET.
Dose-Response Relationship, Drug
Fluorescence Resonance Energy Transfer
Fluorescent Dyes
/ chemical synthesis
Lectins
/ chemistry
Molecular Structure
Polymers
/ chemical synthesis
Spectrometry, Fluorescence
Structure-Activity Relationship
Tryptophan
/ drug effects
Wheat Germ Agglutinins
/ drug effects
FRET
Fluorogenic polymers
Glycoclusters
Glycopolymers
Lectins
Journal
Bioorganic & medicinal chemistry letters
ISSN: 1464-3405
Titre abrégé: Bioorg Med Chem Lett
Pays: England
ID NLM: 9107377
Informations de publication
Date de publication:
15 04 2020
15 04 2020
Historique:
received:
03
12
2019
revised:
31
01
2020
accepted:
09
02
2020
pubmed:
27
2
2020
medline:
1
5
2021
entrez:
27
2
2020
Statut:
ppublish
Résumé
A convenient assembly of fluorogenic glycopolymers having various polymer compositions was accomplished from the corresponding glycomonomer and dansyl monomer by means of radical polymerization, and the water-soluble glycopolymers gave typical fluorescence spectroscopic profiles due to the dansyl moieties on the glycopolymer in aqueous media. Biological evaluation of the polymer against wheat germ agglutinin (WGA) was accomplished on the basis of fluorescence changes due to tryptophan residues on WGA, and the affinities between the glycopolymers and WGA were estimated to be 4.7 × 10
Identifiants
pubmed: 32098722
pii: S0960-894X(20)30094-9
doi: 10.1016/j.bmcl.2020.127024
pii:
doi:
Substances chimiques
Fluorescent Dyes
0
Lectins
0
Polymers
0
Wheat Germ Agglutinins
0
Tryptophan
8DUH1N11BX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
127024Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.