Human auditory ossicles as an alternative optimal source of ancient DNA.
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
10
12
2019
accepted:
11
02
2020
pubmed:
27
2
2020
medline:
25
2
2021
entrez:
27
2
2020
Statut:
ppublish
Résumé
DNA recovery from ancient human remains has revolutionized our ability to reconstruct the genetic landscape of the past. Ancient DNA research has benefited from the identification of skeletal elements, such as the cochlear part of the osseous inner ear, that provides optimal contexts for DNA preservation; however, the rich genetic information obtained from the cochlea must be counterbalanced against the loss of morphological information caused by its sampling. Motivated by similarities in developmental processes and histological properties between the cochlea and auditory ossicles, we evaluate the ossicles as an alternative source of ancient DNA. We show that ossicles perform comparably to the cochlea in terms of DNA recovery, finding no substantial reduction in data quantity and minimal differences in data quality across preservation conditions. Ossicles can be sampled from intact skulls or disarticulated petrous bones without damage to surrounding bone, and we argue that they should be used when available to reduce damage to human remains. Our results identify another optimal skeletal element for ancient DNA analysis and add to a growing toolkit of sampling methods that help to better preserve skeletal remains for future research while maximizing the likelihood that ancient DNA analysis will produce useable results.
Identifiants
pubmed: 32098773
pii: gr.260141.119
doi: 10.1101/gr.260141.119
pmc: PMC7111520
doi:
Substances chimiques
DNA, Ancient
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
427-436Subventions
Organisme : European Research Council
ID : 263441
Pays : International
Informations de copyright
© 2020 Sirak et al.; Published by Cold Spring Harbor Laboratory Press.
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