Human RPA activates BLM's bidirectional DNA unwinding from a nick.
BLM
DNA unwinding
RPA
chicken
helicase
molecular biophysics
optical tweezers
single molecule
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
26 02 2020
26 02 2020
Historique:
received:
02
12
2019
accepted:
25
02
2020
pubmed:
27
2
2020
medline:
10
4
2021
entrez:
27
2
2020
Statut:
epublish
Résumé
BLM is a multifunctional helicase that plays critical roles in maintaining genome stability. It processes distinct DNA substrates, but not nicked DNA, during many steps in DNA replication and repair. However, how BLM prepares itself for diverse functions remains elusive. Here, using a combined single-molecule approach, we find that a high abundance of BLMs can indeed unidirectionally unwind dsDNA from a nick when an external destabilizing force is applied. Strikingly, human replication protein A (hRPA) not only ensures that limited quantities of BLMs processively unwind nicked dsDNA under a reduced force but also permits the translocation of BLMs on both intact and nicked ssDNAs, resulting in a bidirectional unwinding mode. This activation necessitates BLM targeting on the nick and the presence of free hRPAs in solution whereas direct interactions between them are dispensable. Our findings present novel DNA unwinding activities of BLM that potentially facilitate its function switching in DNA repair.
Identifiants
pubmed: 32101168
doi: 10.7554/eLife.54098
pii: 54098
pmc: PMC7065910
doi:
pii:
Substances chimiques
DNA, Single-Stranded
0
Replication Protein A
0
Bloom syndrome protein
EC 3.6.1.-
RecQ Helicases
EC 3.6.4.12
DNA Topoisomerases, Type I
EC 5.99.1.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Science and Technology of the People's Republic of China
ID : 2016YFA0500902
Organisme : Ministry of Science and Technology of the People's Republic of China
ID : 2017YFA0106700
Organisme : Shanghai Natural Science Foundation
ID : 19ZR1434100
Organisme : Institut National Du Cancer
ID : PLBIO2017-167
Organisme : The French National League Against Cancer
ID : EL2028.LNCC/MaM
Organisme : The Natural Science Foundation of Shanghai
ID : 19ZR1434100
Organisme : The French National Cancer Institute
ID : PLBIO2017-167
Informations de copyright
© 2020, Qin et al.
Déclaration de conflit d'intérêts
ZQ, LB, XH, SZ, XZ, YL, ML, MM, XX, BS No competing interests declared
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