PDGFR-β Promoter Forms a Vacancy G-Quadruplex that Can Be Filled in by dGMP: Solution Structure and Molecular Recognition of Guanine Metabolites and Drugs.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
18 03 2020
18 03 2020
Historique:
pubmed:
27
2
2020
medline:
14
5
2021
entrez:
27
2
2020
Statut:
ppublish
Résumé
Aberrant expression of PDGFR-β is associated with a number of diseases. The G-quadruplexes (G4s) formed in PDGFR-β gene promoter are transcriptional modulators and amenable to small molecule targeting. The major G4 formed in the PDGFR-β gene promoter was previously shown to have a broken G-strand. Herein, we report that the PDGFR-β gene promoter sequence forms a vacancy G-quadruplex (vG4) which can be filled in and stabilized by physiologically relevant guanine metabolites, such as dGMP, GMP, and cGMP, as well as guanine-derivative drugs. We determined the NMR structure of the dGMP-fill-in PDGFR-β vG4 in K
Identifiants
pubmed: 32101424
doi: 10.1021/jacs.9b12770
pmc: PMC7241120
mid: NIHMS1587277
doi:
Substances chimiques
Deoxyguanine Nucleotides
0
DNA
9007-49-2
Receptor, Platelet-Derived Growth Factor beta
EC 2.7.10.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5204-5211Subventions
Organisme : NCI NIH HHS
ID : P30 CA023168
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA177585
Pays : United States
Références
Angew Chem Int Ed Engl. 2015 May 4;54(19):5588-91
pubmed: 25775974
Nat Chem. 2013 Mar;5(3):182-6
pubmed: 23422559
J Chem Theory Comput. 2012 Jul 10;8(7):2506-2520
pubmed: 23197943
J Am Chem Soc. 2019 Jul 17;141(28):11059-11070
pubmed: 31283877
Bioessays. 2001 Jun;23(6):494-507
pubmed: 11385629
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14581-6
pubmed: 26553979
Org Lett. 2016 Jul 15;18(14):3398-401
pubmed: 27340903
J Am Chem Soc. 2011 Nov 9;133(44):17673-80
pubmed: 21967482
Nat Rev Cancer. 2015 Sep;15(9):528-39
pubmed: 26299592
ACS Chem Biol. 2019 Sep 20;14(9):1951-1963
pubmed: 31433157
Angew Chem Int Ed Engl. 2018 Aug 20;57(34):10888-10893
pubmed: 29888501
Angew Chem Int Ed Engl. 2010 Mar 15;49(12):2238-41
pubmed: 20186894
J Am Chem Soc. 2019 Nov 13;141(45):18038-18047
pubmed: 31661272
Chem Biol. 2013 Apr 18;20(4):521-32
pubmed: 23601641
Future Med Chem. 2010 Apr;2(4):619-46
pubmed: 20563318
J Mol Graph. 1996 Feb;14(1):33-8, 27-8
pubmed: 8744570
Nucleic Acids Res. 2016 Jan 29;44(2):910-6
pubmed: 26673723
J Am Chem Soc. 2013 Apr 10;135(14):5344-7
pubmed: 23521511
Biochim Biophys Acta Gen Subj. 2018 Apr;1862(4):846-854
pubmed: 29288770
Nat Rev Drug Discov. 2011 Apr;10(4):261-75
pubmed: 21455236
J Biomol NMR. 1995 Jun;5(4):333-8
pubmed: 7647551
FEBS Lett. 1993 Aug 2;327(3):261-4
pubmed: 7688695
J Chem Theory Comput. 2015 Dec 8;11(12):5723-36
pubmed: 26588601
J Chem Theory Comput. 2013 May 14;9(5):2339-2354
pubmed: 24058302
Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12116-21
pubmed: 8901542
Biochemistry. 2010 May 18;49(19):4208-19
pubmed: 20377208
Angew Chem Int Ed Engl. 2016 Oct 24;55(44):13759-13764
pubmed: 27714981
Nat Genet. 2016 Oct;48(10):1267-72
pubmed: 27618450
Nat Chem. 2014 Jan;6(1):75-80
pubmed: 24345950
J Biol Chem. 2012 Dec 7;287(50):41576-82
pubmed: 23048026
ACS Omega. 2019 Feb 28;4(2):2533-2539
pubmed: 30842981
J Am Chem Soc. 2012 Aug 15;134(32):13220-3
pubmed: 22866911
Biochemistry. 2018 Aug 7;57(31):4638-4643
pubmed: 29327580
Angew Chem Int Ed Engl. 2018 Nov 19;57(47):15395-15399
pubmed: 30222243
Angew Chem Int Ed Engl. 2016 Nov 21;55(48):15162-15165
pubmed: 27860177
J Am Chem Soc. 2016 Mar 2;138(8):2563-70
pubmed: 26841249
Nucleic Acids Res. 2015 Oct 15;43(18):8627-37
pubmed: 26350216
Mol Cell Biochem. 1994 Nov 9;140(1):1-22
pubmed: 7877593
J Magn Reson. 2003 Jan;160(1):65-73
pubmed: 12565051
Nature. 2018 Jun;558(7710):465-469
pubmed: 29899445
Nucleic Acids Res. 2013 Dec;41(22):10584-92
pubmed: 24005038
Nat Commun. 2010 Oct 19;1:100
pubmed: 20981028
J Am Chem Soc. 2017 Jun 7;139(22):7456-7475
pubmed: 28471683
J Med Chem. 2016 Jul 14;59(13):5987-6011
pubmed: 26840940