Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia.
Adult
Age Factors
Aged
Aged, 80 and over
Aging
Bacteria
/ classification
DNA, Intergenic
/ genetics
Ecosystem
Feces
/ microbiology
Female
Fungi
/ classification
Gastrointestinal Microbiome
Gastrointestinal Tract
/ microbiology
Humans
Italy
Male
Microbiota
Mouth
/ microbiology
Mycobiome
RNA, Ribosomal, 16S
/ genetics
Sequence Analysis, DNA
Skin
/ microbiology
Young Adult
16S rRNA sequencing
ITS1 sequencing
Sardinian
aging
gut microbiota
human microbiome
oral microbiota
skin microbiota
Journal
mSphere
ISSN: 2379-5042
Titre abrégé: mSphere
Pays: United States
ID NLM: 101674533
Informations de publication
Date de publication:
26 02 2020
26 02 2020
Historique:
entrez:
28
2
2020
pubmed:
28
2
2020
medline:
25
11
2020
Statut:
epublish
Résumé
Human body microbes interact with the host, forming microbial communities that are in continual flux during the aging process. Previous studies have mostly focused on surveying a single body habitat to determine the age-related variation in the bacterial and fungal communities. A more comprehensive understanding of the variation in the human microbiota and mycobiota across multiple body habitats related to aging is still unclear. To obtain an integrated view of the spatial distribution of microbes in a specific Mediterranean population across a wide age range, we surveyed the bacterial and fungal communities in the skin, oral cavity, and gut in the young, elderly, and centenarians in Sardinia using 16S rRNA gene and internal transcribed spacer 1 (ITS1) sequencing. We found that the distribution and correlation of bacterial and fungal communities in Sardinians were largely determined by body site. In each age group, the bacterial and fungal communities found in the skin were significantly different in structure. In the oral cavity, age had a marginal impact on the structures of the bacterial and fungal communities. Furthermore, the gut bacterial communities in centenarians clustered separately from those of the young and elderly, while the fungal communities in the gut habitat could not be separated by host age.
Identifiants
pubmed: 32102941
pii: 5/1/e00558-19
doi: 10.1128/mSphere.00558-19
pmc: PMC7045387
pii:
doi:
Substances chimiques
DNA, Intergenic
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 Wu et al.
Références
Atherosclerosis. 2015 Oct;242(2):418-23
pubmed: 26282947
J Invest Dermatol. 2010 Sep;130(9):2211-21
pubmed: 20463690
PLoS One. 2014 Nov 05;9(11):e112373
pubmed: 25372853
Bioinformatics. 2014 Nov 1;30(21):3123-4
pubmed: 25061070
Appl Environ Microbiol. 2006 Jul;72(7):5069-72
pubmed: 16820507
Genome Biol. 2011;12(5):R50
pubmed: 21624126
Microbiome. 2018 Mar 21;6(1):54
pubmed: 29562943
J Invest Dermatol. 2011 Feb;131(2):382-90
pubmed: 21048787
Nature. 2012 Jun 13;486(7402):207-14
pubmed: 22699609
Microbiome. 2017 Nov 25;5(1):153
pubmed: 29178920
J Nutr Health Aging. 2010 Feb;14(2):97-102
pubmed: 20126955
Cell. 2014 Jan 30;156(3):408-11
pubmed: 24485451
PLoS One. 2010 May 17;5(5):e10667
pubmed: 20498852
Cell. 2013 Jun 6;153(6):1194-217
pubmed: 23746838
J Microbiol Biotechnol. 2015 Aug;25(8):1195-204
pubmed: 25839332
Cell Syst. 2016 Dec 21;3(6):572-584.e3
pubmed: 27818083
Front Microbiol. 2017 Jun 23;8:1162
pubmed: 28690602
BMC Microbiol. 2016 May 25;16:90
pubmed: 27220822
Sci Rep. 2019 Feb 5;9(1):1437
pubmed: 30723224
J Clin Microbiol. 2008 Apr;46(4):1407-17
pubmed: 18216213
Nutr Healthy Aging. 2018 Jun 15;4(4):267-285
pubmed: 29951588
mSphere. 2017 Sep 27;2(5):
pubmed: 28959739
Microbiome. 2014 Feb 24;2(1):6
pubmed: 24558975
Nature. 2012 May 09;486(7402):222-7
pubmed: 22699611
Sci Rep. 2017 Sep 5;7(1):10567
pubmed: 28874721
Science. 2015 Dec 4;350(6265):1214-5
pubmed: 26785481
Nature. 2014 Jan 23;505(7484):559-63
pubmed: 24336217
Curr Biol. 2016 Jun 6;26(11):1480-5
pubmed: 27185560
Cell Host Microbe. 2017 Apr 12;21(4):455-466.e4
pubmed: 28407483
PLoS One. 2015 Oct 28;10(10):e0141842
pubmed: 26510185
ISME J. 2015 Sep;9(9):1979-90
pubmed: 25647347
Nature. 2013 Jun 20;498(7454):367-70
pubmed: 23698366
mSystems. 2019 May 14;4(4):
pubmed: 31098397
Am J Clin Nutr. 1999 May;69(5):1052S-1057S
pubmed: 10232648
Gut. 2018 Dec;67(12):2213-2222
pubmed: 30194220
Sci Rep. 2016 Feb 24;6:22164
pubmed: 26907866
Cell. 2014 Nov 6;159(4):789-99
pubmed: 25417156
Cell. 2016 May 5;165(4):854-66
pubmed: 27153496
Circ Res. 2019 Jan 4;124(1):94-100
pubmed: 30582442
Nature. 2012 Aug 9;488(7410):178-84
pubmed: 22797518
Ann Neurol. 2018 Jul;84(1):23-36
pubmed: 29733457
PeerJ. 2016 Oct 18;4:e2584
pubmed: 27781170
Nature. 2012 Sep 13;489(7415):242-9
pubmed: 22972297
Cell. 2016 Nov 17;167(5):1339-1353.e21
pubmed: 27863247
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
ISME J. 2016 Jan;10(1):170-82
pubmed: 26090993
Cell Host Microbe. 2019 Aug 14;26(2):265-272.e4
pubmed: 31324413
Nat Rev Microbiol. 2011 Apr;9(4):244-53
pubmed: 21407241
Science. 2009 Dec 18;326(5960):1694-7
pubmed: 19892944
J Invest Dermatol. 1979 Jul;73(1):108-11
pubmed: 448169
Nat Commun. 2018 Apr 19;9(1):1557
pubmed: 29674608
Front Microbiol. 2016 Mar 24;7:377
pubmed: 27047484
Environ Microbiol. 2015 Mar;17(3):699-710
pubmed: 24800728
Wound Repair Regen. 2016 Jan-Feb;24(1):163-74
pubmed: 26463872
Microbiome. 2016 Aug 24;4(1):46
pubmed: 27558504
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Curr Biol. 2016 Sep 26;26(18):R832-R833
pubmed: 27676296
Cell. 2017 Jun 15;169(7):1249-1262.e13
pubmed: 28622510
Immunity. 2015 May 19;42(5):805-13
pubmed: 25992857
Mol Ecol. 2013 Nov;22(21):5271-7
pubmed: 24112409
Elife. 2017 Aug 22;6:
pubmed: 28826469
Science. 2016 Apr 29;352(6285):560-4
pubmed: 27126039
Sci Rep. 2018 Jan 11;8(1):414
pubmed: 29323208
Med Mycol. 2004 Feb;42(1):35-42
pubmed: 14982112
Nature. 2016 Jul 06;535(7610):56-64
pubmed: 27383980
Gut. 2001 Feb;48(2):198-205
pubmed: 11156640
Aging (Milano). 1999 Jun;11(3):142-9
pubmed: 10476308
PLoS One. 2013 Jun 17;8(6):e66019
pubmed: 23799070
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4586-91
pubmed: 20571116
mSystems. 2019 Jul 9;4(4):
pubmed: 31289141
Nature. 2014 Oct 2;514(7520):59-64
pubmed: 25279917
Nature. 2018 Mar 8;555(7695):210-215
pubmed: 29489753
N Engl J Med. 2016 Dec 15;375(24):2369-2379
pubmed: 27974040