Zinc can counteract selection for ciprofloxacin resistance.


Journal

FEMS microbiology letters
ISSN: 1574-6968
Titre abrégé: FEMS Microbiol Lett
Pays: England
ID NLM: 7705721

Informations de publication

Date de publication:
01 02 2020
Historique:
received: 14 01 2020
accepted: 26 02 2020
pubmed: 28 2 2020
medline: 4 2 2021
entrez: 28 2 2020
Statut: ppublish

Résumé

Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-characterized, experimental studies exploring their combined effects on AMR evolution are rare. It has previously been demonstrated that fluoroquinolone antibiotics such as ciprofloxacin can chelate metal ions. To investigate how ciprofloxacin resistance is affected by the presence of metals, we quantified selection dynamics between a ciprofloxacin-susceptible and a ciprofloxacin-resistant Escherichia coli strain across a gradient of ciprofloxacin concentrations in presence and absence of zinc. The presence of zinc reduced growth of both strains, while ciprofloxacin inhibited exclusively the susceptible one. When present in combination zinc retained its inhibitory effect, while ciprofloxacin inhibition of the susceptible strain was reduced. Consequently, the minimal selective concentration for ciprofloxacin resistance increased up to five-fold in the presence of zinc. Environmental pollution usually comprises complex mixtures of antimicrobial agents. In addition to the usual focus on additive or synergistic interactions in complex selective mixtures, our findings highlight the importance of antagonistic selective interactions when considering resistance evolution.

Identifiants

pubmed: 32105320
pii: 5762671
doi: 10.1093/femsle/fnaa038
pmc: PMC7082703
pii:
doi:

Substances chimiques

Anti-Infective Agents 0
Ciprofloxacin 5E8K9I0O4U
Zinc J41CSQ7QDS

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Medical Research Council
ID : MR/N007174/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S037713/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom

Informations de copyright

© FEMS 2020.

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Auteurs

Michiel Vos (M)

European Centre for Environment and Human Health, University of Exeter Medical School, Knowledge Spa, Royal Cornwall Hospital Truro, TR1 3HDTruro, Cornwall, UK.

Louise Sibleyras (L)

European Centre for Environment and Human Health, University of Exeter Medical School, Knowledge Spa, Royal Cornwall Hospital Truro, TR1 3HDTruro, Cornwall, UK.
Université Paris Saclay, Department of Biology, Espace Technologique Bat. Discovery - RD 128 - 2e ét, 91190 Saint-Aubin, France.

Lai Ka Lo (LK)

European Centre for Environment and Human Health, University of Exeter Medical School, Knowledge Spa, Royal Cornwall Hospital Truro, TR1 3HDTruro, Cornwall, UK.
Institute for Evolution & Biodiversity, Universität Münster, Hüfferstraße 1, 48149 Münster, Germany.
College of Life and Environmental Science, University of Exeter, Penryn Campus, TR10 9FE Penryn, Cornwall, UK.

Elze Hesse (E)

College of Life and Environmental Science, University of Exeter, Penryn Campus, TR10 9FE Penryn, Cornwall, UK.
Environment and Sustainability Institute, University of Exeter, Penryn Campus, TR10 9FE Penryn, Cornwall, UK.

William Gaze (W)

European Centre for Environment and Human Health, University of Exeter Medical School, Knowledge Spa, Royal Cornwall Hospital Truro, TR1 3HDTruro, Cornwall, UK.

Uli Klümper (U)

European Centre for Environment and Human Health, University of Exeter Medical School, Knowledge Spa, Royal Cornwall Hospital Truro, TR1 3HDTruro, Cornwall, UK.
College of Life and Environmental Science, University of Exeter, Penryn Campus, TR10 9FE Penryn, Cornwall, UK.
Environment and Sustainability Institute, University of Exeter, Penryn Campus, TR10 9FE Penryn, Cornwall, UK.

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Classifications MeSH