Comparison of various radioactive payloads for a human monoclonal antibody to glycoprotein 41 for elimination of HIV-infected cells.
177-Lutetium
213-Bismuth
225-Actinium
Human monocytes
Human peripheral blood mononuclear cells
Radioimmunotherapy
Journal
Nuclear medicine and biology
ISSN: 1872-9614
Titre abrégé: Nucl Med Biol
Pays: United States
ID NLM: 9304420
Informations de publication
Date de publication:
Historique:
received:
08
10
2019
revised:
10
02
2020
accepted:
16
02
2020
pubmed:
1
3
2020
medline:
7
5
2021
entrez:
1
3
2020
Statut:
ppublish
Résumé
cART has significantly improved the life expectancy of people living with HIV (PLWH). However, it fails to eliminate the long-lived reservoir of latent HIV-infected cells. Radioimmunotherapy (RIT) relies on antigen-specific monoclonal antibodies (mAbs) for targeted delivery of lethal doses of ionizing radiation to cells. Previously, we have demonstrated that human mAb 2556 against HIV gp41 conjugated with In this study we have conjugated 2556 mAb with After three days post-treatment of infected PBMCs and monocytes, These results indicate that RIT holds promise as a novel treatment option for the eradication of HIV-infected cells that merits further study in combination with cART and reactivation drugs.
Sections du résumé
BACKGROUND
cART has significantly improved the life expectancy of people living with HIV (PLWH). However, it fails to eliminate the long-lived reservoir of latent HIV-infected cells. Radioimmunotherapy (RIT) relies on antigen-specific monoclonal antibodies (mAbs) for targeted delivery of lethal doses of ionizing radiation to cells. Previously, we have demonstrated that human mAb 2556 against HIV gp41 conjugated with
METHODS
In this study we have conjugated 2556 mAb with
RESULTS
After three days post-treatment of infected PBMCs and monocytes,
CONCLUSION
These results indicate that RIT holds promise as a novel treatment option for the eradication of HIV-infected cells that merits further study in combination with cART and reactivation drugs.
Identifiants
pubmed: 32113033
pii: S0969-8051(19)30510-4
doi: 10.1016/j.nucmedbio.2020.02.009
pmc: PMC7195259
mid: NIHMS1567452
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Membrane Glycoproteins
0
glycoprotein 41
0
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
80-88Subventions
Organisme : NIAID NIH HHS
ID : P30 AI124414
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA048609
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH112391
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS098956
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflicts of interest.
Références
Neurotox Res. 2016 Nov;30(4):677-697
pubmed: 27364698
Oncoimmunology. 2019 May 1;8(8):1607673
pubmed: 31413916
Curr Opin Virol. 2016 Jun;18:50-6
pubmed: 27023283
J Neurovirol. 2002 Dec;8 Suppl 2:115-21
pubmed: 12491162
Anal Chem. 2005 Oct 1;77(19):6288-91
pubmed: 16194090
Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21105-10
pubmed: 22160723
PLoS Med. 2006 Nov;3(11):e427
pubmed: 17090209
Clin Chim Acta. 2016 May 1;456:128-136
pubmed: 26940950
Pharmaceutics. 2018 Dec 05;10(4):
pubmed: 30563123
Nat Rev Cancer. 2015 Jun;15(6):347-60
pubmed: 25998714
Curr Opin HIV AIDS. 2013 May;8(3):217-23
pubmed: 23478911
J Clin Oncol. 2014 Sep 1;32(25):2750-7
pubmed: 25024070
Cell Immunol. 2011;267(2):109-23
pubmed: 21292246
Front Med (Lausanne). 2016 Sep 26;3:41
pubmed: 27725930
N Engl J Med. 2005 Feb 3;352(5):441-9
pubmed: 15689582
Curr Radiopharm. 2018;11(3):192-199
pubmed: 29793418
Nat Biotechnol. 2018 Dec 6;36(12):1125-1127
pubmed: 30520873
J Nucl Med. 2009 Dec;50(12):2033-41
pubmed: 19910417
Int J Radiat Oncol Biol Phys. 2004 Aug 1;59(5):1269-71
pubmed: 15275708
Immunity. 2018 May 15;48(5):872-895
pubmed: 29768175
PLoS One. 2013 Jul 26;8(7):e69270
pubmed: 23922698
AIDS. 2016 Feb 20;30(4):563-72
pubmed: 26595540
Neoplasma. 2017;64(5):641-654
pubmed: 28592116
PLoS One. 2012;7(3):e31866
pubmed: 22427811
Immunol Cell Biol. 2012 Jan;90(1):47-54
pubmed: 22083528
Cell Stem Cell. 2012 Feb 3;10(2):137-47
pubmed: 22305563