Fecal Serine Protease Profiling in Inflammatory Bowel Diseases.
Holobiont
fecal proteases
gut microbiota
inflammatory bowel diseases
microbiome
protease profiling
serine protease inhibitors
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
10
2019
accepted:
14
01
2020
entrez:
3
3
2020
pubmed:
3
3
2020
medline:
22
6
2021
Statut:
epublish
Résumé
Serine proteases are extensively known to play key roles in many physiological processes. However, their dysregulation is often associated to several diseases including inflammatory bowel diseases (IBD). Here, we used specific substrates to monitor fecal protease activities in a large cohort of healthy and IBD patients. Of interest, serine protease activity was 10-fold higher in IBD fecal samples compared to healthy controls. Moreover, functional analysis of these fecal proteolytic activities revealed that the most increased activities are trypsin-like, elastase-like and cathepsin G-like. We also show for the first time, an increase of proteinase 3-like activity in these samples compared to controls. Results presented here will guide further investigations to better understand the relevance of these peptidases in IBD.
Identifiants
pubmed: 32117798
doi: 10.3389/fcimb.2020.00021
pmc: PMC7011180
doi:
Substances chimiques
Serine Proteases
EC 3.4.-
Pancreatic Elastase
EC 3.4.21.36
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21Informations de copyright
Copyright © 2020 Jablaoui, Kriaa, Mkaouar, Akermi, Soussou, Wysocka, Wołoszyn, Amouri, Gargouri, Maguin, Lesner and Rhimi.
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