Transient genome-wide interactions of the master transcription factor NLP7 initiate a rapid nitrogen-response cascade.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
02 03 2020
02 03 2020
Historique:
received:
03
07
2019
accepted:
07
02
2020
entrez:
4
3
2020
pubmed:
4
3
2020
medline:
23
5
2020
Statut:
epublish
Résumé
Dynamic reprogramming of gene regulatory networks (GRNs) enables organisms to rapidly respond to environmental perturbation. However, the underlying transient interactions between transcription factors (TFs) and genome-wide targets typically elude biochemical detection. Here, we capture both stable and transient TF-target interactions genome-wide within minutes after controlled TF nuclear import using time-series chromatin immunoprecipitation (ChIP-seq) and/or DNA adenine methyltransferase identification (DamID-seq). The transient TF-target interactions captured uncover the early mode-of-action of NIN-LIKE PROTEIN 7 (NLP7), a master regulator of the nitrogen signaling pathway in plants. These transient NLP7 targets captured in root cells using temporal TF perturbation account for 50% of NLP7-regulated genes not detectably bound by NLP7 in planta. Rapid and transient NLP7 binding activates early nitrogen response TFs, which we validate to amplify the NLP7-initiated transcriptional cascade. Our approaches to capture transient TF-target interactions genome-wide can be applied to validate dynamic GRN models for any pathway or organism of interest.
Identifiants
pubmed: 32123177
doi: 10.1038/s41467-020-14979-6
pii: 10.1038/s41467-020-14979-6
pmc: PMC7052136
doi:
Substances chimiques
Arabidopsis Proteins
0
NIN-LIKE PROTEIN7, Arabidopsis
0
Transcription Factors
0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1157Subventions
Organisme : NIGMS NIH HHS
ID : F32 GM116347
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM032877
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM121753
Pays : United States
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