Comprehensive fitness maps of Hsp90 show widespread environmental dependence.
Adaptation, Physiological
Epistasis, Genetic
Gene Expression Regulation, Fungal
/ physiology
Gene-Environment Interaction
Genetic Fitness
HSP90 Heat-Shock Proteins
/ genetics
Models, Molecular
Mutation
Protein Conformation
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Stress, Physiological
Hsp90
S. cerevisiae
deep mutational scanning
evolutionary biology
fitness map
genetics
genomics
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
04 03 2020
04 03 2020
Historique:
received:
21
11
2019
accepted:
03
03
2020
pubmed:
5
3
2020
medline:
5
5
2021
entrez:
5
3
2020
Statut:
epublish
Résumé
Gene-environment interactions have long been theorized to influence molecular evolution. However, the environmental dependence of most mutations remains unknown. Using deep mutational scanning, we engineered yeast with all 44,604 single codon changes encoding 14,160 amino acid variants in Hsp90 and quantified growth effects under standard conditions and under five stress conditions. To our knowledge, these are the largest determined comprehensive fitness maps of point mutants. The growth of many variants differed between conditions, indicating that environment can have a large impact on Hsp90 evolution. Multiple variants provided growth advantages under individual conditions; however, these variants tended to exhibit growth defects in other environments. The diversity of Hsp90 sequences observed in extant eukaryotes preferentially contains variants that supported robust growth under all tested conditions. Rather than favoring substitutions in individual conditions, the long-term selective pressure on Hsp90 may have been that of fluctuating environments, leading to robustness under a variety of conditions.
Identifiants
pubmed: 32129763
doi: 10.7554/eLife.53810
pii: 53810
pmc: PMC7069724
doi:
pii:
Substances chimiques
HSP90 Heat-Shock Proteins
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : EMBO Installation Grant
ID : IG4152
Organisme : Fundação para a Ciência e a Tecnologia
ID : JPIAMR/0001/2016
Organisme : NIGMS NIH HHS
ID : R01 GM112844
Pays : United States
Organisme : ERC Starting Grant
ID : 804569-FIT2GO
Organisme : NIGMS NIH HHS
ID : F32 GM119205
Pays : United States
Informations de copyright
© 2020, Flynn et al.
Déclaration de conflit d'intérêts
JF, AR, PC, IF, DM, CH, CB, DB No competing interests declared
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