The WRKY6 transcription factor affects seed oil accumulation and alters fatty acid compositions in Arabidopsis thaliana.
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
12
08
2019
revised:
13
02
2020
accepted:
28
02
2020
pubmed:
5
3
2020
medline:
25
9
2020
entrez:
5
3
2020
Statut:
ppublish
Résumé
In rapeseed, the oil content of the seed not only supplies energy for seed germination and seedling development but also provides essential dietary nutrients for humans and livestock. Recent studies have revealed that many transcription factors (TFs) regulate the accumulation of fatty acids (FAs) during seed development. WRKY6, a WRKY6 family TF, was reported to serve a function in the plant senescence processes, pathogen defense mechanisms and abiotic stress responses. However, the precise role of WRKY6 in influencing FA accumulation in seeds is still unknown. In this study, we demonstrate that WRKY6 has a high expression level in developing seeds and plays an essential role in regulating the accumulation of FAs in developing seeds of Arabidopsis. Mutation of WRKY6 resulted in significant increase in seed size, accompanied by an increase in FA content and changes in FA composition. Ultrastructure analyses showed that the absence of WRKY6 resulted in more and higher percentage of oil body in the cell of mature seeds. Quantitative real-time PCR analysis revealed changes in the expression of several genes related to photosynthesis and FA biosynthesis in wrky6 mutants at 10 or 16 days after pollination. These results reveal a novel function of WRKY6 influencing seed oil content and FAs compositions. This gene could be used as a promising gene resource to improve FA accumulation and seed yield in Brassica napus through genetic manipulation.
Substances chimiques
Arabidopsis Proteins
0
Fatty Acids
0
Plant Oils
0
Transcription Factors
0
WRKY6 protein, Arabidopsis
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
612-624Subventions
Organisme : China Postdoctoral Science Foundation
ID : 2018M632476
Organisme : Major State Basic Research Development Program of China
ID : 2015CB150200
Organisme : National Natural Science Foundation of China
ID : 31529001
Organisme : National Natural Science Foundation of China
ID : 31570183
Organisme : National Natural Science Foundation of China
ID : 31661143004
Organisme : the National Key R & D Program of China
ID : 2016YFD0100701
Informations de copyright
© 2020 Scandinavian Plant Physiology Society.
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