Ratiometric population sensing by a pump-probe signaling system in Bacillus subtilis.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
04 03 2020
04 03 2020
Historique:
received:
22
02
2019
accepted:
04
02
2020
entrez:
6
3
2020
pubmed:
7
3
2020
medline:
27
6
2020
Statut:
epublish
Résumé
Communication by means of diffusible signaling molecules facilitates higher-level organization of cellular populations. Gram-positive bacteria frequently use signaling peptides, which are either detected at the cell surface or 'probed' by intracellular receptors after being pumped into the cytoplasm. While the former type is used to monitor cell density, the functions of pump-probe networks are less clear. Here we show that pump-probe networks can, in principle, perform different tasks and mediate quorum-sensing, chronometric and ratiometric control. We characterize the properties of the prototypical PhrA-RapA system in Bacillus subtilis using FRET. We find that changes in extracellular PhrA concentrations are tracked rather poorly; instead, cells accumulate and strongly amplify the signal in a dose-dependent manner. This suggests that the PhrA-RapA system, and others like it, have evolved to sense changes in the composition of heterogeneous populations and infer the fraction of signal-producing cells in a mixed population to coordinate cellular behaviors.
Identifiants
pubmed: 32132526
doi: 10.1038/s41467-020-14840-w
pii: 10.1038/s41467-020-14840-w
pmc: PMC7055314
doi:
Substances chimiques
ATP-Binding Cassette Transporters
0
Bacterial Proteins
0
rapA protein, Bacillus subtilis
147605-09-2
Phosphoprotein Phosphatases
EC 3.1.3.16
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1176Références
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