Improving glucose and xylose assimilation in Azotobacter vinelandii by adaptive laboratory evolution.
Adaptive laboratory evolution
Azotobacter vinelandii
Glucose–xylose assimilation
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
05 Mar 2020
05 Mar 2020
Historique:
received:
02
10
2019
accepted:
27
02
2020
entrez:
7
3
2020
pubmed:
7
3
2020
medline:
24
3
2020
Statut:
epublish
Résumé
Azotobacter vinelandii is a microorganism with biotechnological potential because its ability to produce alginate and polyhydroxybutyrate. Large-scale biotechnological processes are oriented to sustainable production by using biomass hydrolysates that are mainly composed by glucose and xylose. In the present study, it was observed that A. vinelandii was unable to consume xylose as the sole carbon source and that glucose assimilation in the presence of xylose was negatively affected. Adaptive Laboratory Evolution (ALE) was used as a metabolic engineering tool in A. vinelandii, to improve both carbohydrate assimilation. As a result of ALE process, the CT387 strain was obtained. The evolved strain (CT387) grown in shaken flask cultivations with xylose (8 g L
Identifiants
pubmed: 32140791
doi: 10.1007/s11274-020-02822-5
pii: 10.1007/s11274-020-02822-5
doi:
Substances chimiques
Bacterial Proteins
0
Culture Media
0
RNA, Bacterial
0
Xylose
A1TA934AKO
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
46Subventions
Organisme : CONACYT-FONCICYT
ID : 277600
Organisme : DGAPA-UNAM
ID : AG200219
Références
J Bacteriol. 2009 Jul;191(14):4534-45
pubmed: 19429624
Science. 2014 Oct 3;346(6205):75-8
pubmed: 25278608
J Appl Microbiol. 2018 Jul;125(1):181-189
pubmed: 29573518
Microbiology. 2004 May;150(Pt 5):1117-1119
pubmed: 15133068
Mol Microbiol. 2015 Oct;98(3):553-70
pubmed: 26194109
Metab Eng. 2018 Jul;48:94-108
pubmed: 29864584
Microbiology. 2009 Apr;155(Pt 4):1351-1359
pubmed: 19332836
J Bacteriol. 1985 Aug;163(2):528-33
pubmed: 4019408
Microb Ecol. 2012 Feb;63(2):239-48
pubmed: 21811795
Appl Environ Microbiol. 2010 Jul;76(13):4158-68
pubmed: 20435762
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Nucleic Acids Res. 1992 Sep 25;20(18):4940
pubmed: 1383933
Microb Biotechnol. 2014 May;7(3):196-208
pubmed: 24576157
Biochem Eng J. 2001 Mar;7(2):157-162
pubmed: 11173305
Microb Cell Fact. 2013 Jul 01;12:64
pubmed: 23815749
Appl Environ Microbiol. 1995 Feb;61(2):430-3
pubmed: 16534925
Enzyme Microb Technol. 2000 Sep 1;27(6):390-398
pubmed: 10938418
Sci Rep. 2017 Apr 12;7(1):858
pubmed: 28404995
Science. 2016 Aug 5;353(6299):583-6
pubmed: 27493184
Curr Opin Biotechnol. 2011 Aug;22(4):590-4
pubmed: 21497080
Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):7349-7354
pubmed: 28655843
J Bacteriol. 2009 May;191(9):3142-8
pubmed: 19270099
Bioresour Technol. 2017 Dec;245(Pt B):1377-1385
pubmed: 28552568
Microbiology. 2011 Jun;157(Pt 6):1685-1693
pubmed: 21454367
Bioresour Technol. 2008 Nov;99(17):8042-8
pubmed: 18474421