An unusual metal-bound 4-fluorothreonine transaldolase from Streptomyces sp. MA37 catalyses promiscuous transaldol reactions.
4-fluorothreonine
4-fluorothreonine transaldolase
Streptomyces sp. MA37
Transaldolation
β-Hydroxy-α-amino acids
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
11
09
2019
accepted:
23
02
2020
revised:
13
02
2020
pubmed:
7
3
2020
medline:
9
1
2021
entrez:
7
3
2020
Statut:
ppublish
Résumé
β-Hydroxy-α-amino acids (βH-AAs) are key components of many bioactive molecules as well as exist as specialised metabolites. Among these βH-AAs, 4-fluorothreonine (4-FT) is the only naturally occurring fluorinated AA discovered thus far. Here we report overexpression and biochemical characterisation of 4-fluorothreonine transaldolase from Streptomyces sp. MA37 (FTaseMA), a homologue of FTase previously identified in the biosynthesis of 4-FT in S. cattleya. FTaseMA displays considerable substrate plasticity to generate 4-FT as well as other β-hydroxy-α-amino acids with various functionalities at C4 position, giving the prospect of new chemo-enzymatic applications. The enzyme has a hybrid of two catalytic domains, serine hydroxymethyltransferase (S) and aldolase (A). Site-directed mutagenesis allowed the identification of the key residues of FTases, suggesting that the active site of A domain has a historical reminiscent feature in metal-dependent aldolases. Elemental analysis demonstrated that FTaseMA is indeed a Zn
Identifiants
pubmed: 32140842
doi: 10.1007/s00253-020-10497-z
pii: 10.1007/s00253-020-10497-z
pmc: PMC7162832
doi:
Substances chimiques
Amino Acids, Aromatic
0
Bacterial Proteins
0
4-fluorothreonine
102130-93-8
Threonine
2ZD004190S
Transaldolase
EC 2.2.1.2
Zinc
J41CSQ7QDS
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3885-3896Subventions
Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : 31570033, 31811530299, and 31870035
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P00380X/1
Pays : United Kingdom
Organisme : Leverhulme Trust
ID : RPG-2014-418
Organisme : Medical Research Council
ID : MR/S00520X/1
Pays : United Kingdom
Organisme : Royal Asiatic Society of Great Britain and Ireland
ID : IEC\NSFC\170617
Organisme : Leverhulme Trust
ID : AA090088
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