Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products.
Hydrophobic interaction chromatography (HIC)
Ion-exchange chromatography (IEX)
Mass spectrometry
Multi-Dimensional liquid chromatography
Size exclusion chromatography (SEC)
Volatile buffer
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
05 Jun 2020
05 Jun 2020
Historique:
received:
18
12
2019
revised:
21
02
2020
accepted:
22
02
2020
pubmed:
7
3
2020
medline:
2
3
2021
entrez:
7
3
2020
Statut:
ppublish
Résumé
The hyphenation of non-denaturing liquid chromatographic (LC) modes (ion exchange (IEX), size exclusion (SEC) and hydrophobic interaction chromatography (HIC)) with mass spectrometry (MS) has attracted significant attention in the last few years. The inherent problem of these couplings is that non-denaturing LC separations have tended to use non-volatile mobile phase additives. Indeed, classical methods have not been directly compatible with MS. Therefore two approaches can be used to address this challenge: (1) finding innovative volatile mobile phases or (2) adding a desalting step prior to MS detection via the use of multidimensional LC. These two possibilities have been applied to the characterization of charge-, size- and hydrophobic variants of various monoclonal antibodies (mAbs) and related products and have been reviewed in this paper.
Identifiants
pubmed: 32143115
pii: S0731-7085(19)33074-2
doi: 10.1016/j.jpba.2020.113207
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Buffers
0
Volatile Organic Compounds
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
113207Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.