5' tRNA halves are highly expressed in the primate hippocampus and might sequence-specifically regulate gene expression.
gene regulation
k-mer mapping
small noncoding RNAs
tRNA fragments
target identification
target prediction
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
19
09
2019
accepted:
02
03
2020
pubmed:
8
3
2020
medline:
17
6
2020
entrez:
8
3
2020
Statut:
ppublish
Résumé
Fragments of mature tRNAs have long been considered as mere degradation products without physiological function. However, recent reports show that tRNA-derived small RNAs (tsRNAs) play prominent roles in diverse cellular processes across a wide spectrum of species. Contrasting the situation in other small RNA pathways the mechanisms behind these effects appear more diverse, more complex, and are generally less well understood. In addition, surprisingly little is known about the expression profiles of tsRNAs across different tissues and species. Here, we provide an initial overview of tsRNA expression in different species and tissues, revealing very high levels of 5' tRNA halves (5' tRHs) particularly in the primate hippocampus. We further modulated the regulation capacity of selected 5' tRHs in human cells by transfecting synthetic tsRNA mimics ("overexpression") or antisense-RNAs ("inhibition") and identified differentially expressed transcripts based on RNA-seq. We then used a novel
Identifiants
pubmed: 32144192
pii: rna.073395.119
doi: 10.1261/rna.073395.119
pmc: PMC7266158
doi:
Substances chimiques
MicroRNAs
0
RNA, Small Interfering
0
RNA, Small Untranslated
0
RNA, Transfer
9014-25-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
694-707Subventions
Organisme : NINDS NIH HHS
ID : R24 NS092988
Pays : United States
Informations de copyright
© 2020 Jehn et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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