Diatoms synthesize sterols by inclusion of animal and fungal genes in the plant pathway.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 03 2020
06 03 2020
Historique:
received:
30
09
2019
accepted:
10
02
2020
entrez:
8
3
2020
pubmed:
8
3
2020
medline:
18
11
2020
Statut:
epublish
Résumé
Diatoms are ubiquitous microalgae that have developed remarkable metabolic plasticity and gene diversification. Here we report the first elucidation of the complete biosynthesis of sterols in the lineage. The study has been carried out on the bloom-forming species Skeletonema marinoi and Cyclotella cryptica that synthesise an ensemble of sterols with chemotypes of animals (cholesterol and desmosterol), plants (dihydrobrassicasterol and 24-methylene cholesterol), algae (fucosterol) and marine invertebrates (clionasterol). In both species, sterols derive from mevalonate through cyclization of squalene to cycloartenol by cycloartenol synthase. The pathway anticipates synthesis of cholesterol by enzymes of the phytosterol route in plants, as recently reported in Solanaceae. Major divergences stem from reduction of Δ24(28) and Δ24(25) double bonds which, in diatoms, are apparently dependent on sterol reductases of fungi, algae and animals. Phylogenetic comparison revealed a good level of similarity between the sterol biosynthetic genes of S. marinoi and C. cryptica with those in the genomes of the other diatoms sequenced so far.
Identifiants
pubmed: 32144288
doi: 10.1038/s41598-020-60993-5
pii: 10.1038/s41598-020-60993-5
pmc: PMC7060231
doi:
Substances chimiques
Phytosterols
0
Sterols
0
Triterpenes
0
Squalene
7QWM220FJH
Intramolecular Transferases
EC 5.4.-
cycloartenol synthase
EC 5.4.99.8
Mevalonic Acid
S5UOB36OCZ
cycloartenol
YU32VE82N3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4204Références
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