DNA Methylation-Mediated Modulation of Endocytosis as Potential Mechanism for Synaptic Function Regulation in Murine Inhibitory Cortical Interneurons.
Animals
Clathrin
Cytoskeletal Proteins
/ genetics
DNA (Cytosine-5-)-Methyltransferase 1
/ genetics
DNA Methylation
/ genetics
DNA-Binding Proteins
/ genetics
Dioxygenases
/ genetics
Endocytosis
/ genetics
Epigenome
Epilepsy, Temporal Lobe
/ genetics
GABAergic Neurons
/ metabolism
Humans
Inhibitory Postsynaptic Potentials
Interneurons
/ metabolism
Intracellular Signaling Peptides and Proteins
/ genetics
Mice
Neural Inhibition
/ genetics
Patch-Clamp Techniques
Proto-Oncogene Proteins
/ genetics
Reverse Transcriptase Polymerase Chain Reaction
Synapses
/ metabolism
Synaptic Vesicles
/ metabolism
Transcriptome
DNMT1
GABA
TET
cortical inhibition
synaptic function
temporal lobe epilepsy
Journal
Cerebral cortex (New York, N.Y. : 1991)
ISSN: 1460-2199
Titre abrégé: Cereb Cortex
Pays: United States
ID NLM: 9110718
Informations de publication
Date de publication:
01 06 2020
01 06 2020
Historique:
received:
05
09
2019
revised:
14
12
2019
accepted:
10
01
2020
pubmed:
10
3
2020
medline:
17
12
2021
entrez:
10
3
2020
Statut:
ppublish
Résumé
The balance of excitation and inhibition is essential for cortical information processing, relying on the tight orchestration of the underlying subcellular processes. Dynamic transcriptional control by DNA methylation, catalyzed by DNA methyltransferases (DNMTs), and DNA demethylation, achieved by ten-eleven translocation (TET)-dependent mechanisms, is proposed to regulate synaptic function in the adult brain with implications for learning and memory. However, focus so far is laid on excitatory neurons. Given the crucial role of inhibitory cortical interneurons in cortical information processing and in disease, deciphering the cellular and molecular mechanisms of GABAergic transmission is fundamental. The emerging relevance of DNMT and TET-mediated functions for synaptic regulation irrevocably raises the question for the targeted subcellular processes and mechanisms. In this study, we analyzed the role dynamic DNA methylation has in regulating cortical interneuron function. We found that DNMT1 and TET1/TET3 contrarily modulate clathrin-mediated endocytosis. Moreover, we provide evidence that DNMT1 influences synaptic vesicle replenishment and GABAergic transmission, presumably through the DNA methylation-dependent transcriptional control over endocytosis-related genes. The relevance of our findings is supported by human brain sample analysis, pointing to a potential implication of DNA methylation-dependent endocytosis regulation in the pathophysiology of temporal lobe epilepsy, a disease characterized by disturbed synaptic transmission.
Identifiants
pubmed: 32147726
pii: 5799017
doi: 10.1093/cercor/bhaa009
pmc: PMC7264686
doi:
Substances chimiques
Clathrin
0
Cytoskeletal Proteins
0
DNA-Binding Proteins
0
Intracellular Signaling Peptides and Proteins
0
Proto-Oncogene Proteins
0
TET1 protein, mouse
0
WIPF1 protein, human
0
Dioxygenases
EC 1.13.11.-
Tet3 protein, mouse
EC 1.13.11.-
DNA (Cytosine-5-)-Methyltransferase 1
EC 2.1.1.37
Dnmt1 protein, mouse
EC 2.1.1.37
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3921-3937Informations de copyright
© The Author(s) 2020. Published by Oxford University Press.
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