Immunochemical approaches for detection of aflatoxin B1 in herbal medicines.

aflatoxin B1 fluorescent polarisation immunoassay herbal medicine membrane-based flow-through rapid test

Journal

Phytochemical analysis : PCA
ISSN: 1099-1565
Titre abrégé: Phytochem Anal
Pays: England
ID NLM: 9200492

Informations de publication

Date de publication:
Sep 2020
Historique:
received: 28 08 2019
revised: 24 01 2020
accepted: 24 01 2020
pubmed: 10 3 2020
medline: 5 8 2020
entrez: 10 3 2020
Statut: ppublish

Résumé

Aflatoxin B1 (AFB1) is a toxic low-molecular-weight secondary metabolite of Aspergillus flavus and A. parasiticus. AFB1 was classified as a Group I carcinogen by the World Health Organisation for Research on Cancer in 1993. AFB1 is an unavoidable natural contaminant of some herbal medicine, able to cause serious health issues for humans consuming the related medicine. Therefore, this study aimed to develop an efficient fluorescence polarisation immunoassay (FPIA) and a rapid, low-cost, and easy-to-use membrane-based flow-through immunoassay (MBA) for determination of AFB1 in herbal medicine Origanum vulgare L., Rubus idaeus L., Urtica dioica L. and Sorbus aucuparia L. A cut-off level of the developed MBA was 0.8 ppb. Validation of the developed test was performed with blank and spiked samples. Using three naturally contaminated or three artificially spiked samples. The FPIA showed a linear working range of 8.6 to 64 ppb, and a half maximal inhibitory concentration (IC The results were in good correlation with the enzymelinked immunosorbent assay (ELISA) results (the IC

Identifiants

pubmed: 32150783
doi: 10.1002/pca.2931
doi:

Substances chimiques

Aflatoxin B1 9N2N2Y55MH

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

662-669

Subventions

Organisme : Ministry of Education and Science of the Russian Federation
ID : RFMEFI60417X0198

Informations de copyright

© 2020 John Wiley & Sons, Ltd.

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Auteurs

Anna Raysyan (A)

Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.

Sergei A Eremin (SA)

Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia.

Natalia V Beloglazova (NV)

Faculty of Pharmaceutical Sciences, Centre of Excellence in Mycotoxicology and Public Health, Ghent University, Ghent, Belgium.
Nanotechnology Education and Research Center, South Ural State University, Chelyabinsk, Russia.

Sarah De Saeger (S)

Faculty of Pharmaceutical Sciences, Centre of Excellence in Mycotoxicology and Public Health, Ghent University, Ghent, Belgium.

Irina V Gravel (IV)

Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.

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