Structural insights into influenza A virus ribonucleoproteins reveal a processive helical track as transcription mechanism.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
19
10
2018
accepted:
24
01
2020
pubmed:
11
3
2020
medline:
28
7
2020
entrez:
11
3
2020
Statut:
ppublish
Résumé
The influenza virus genome consists of eight viral ribonucleoproteins (vRNPs), each consisting of a copy of the polymerase, one of the genomic RNA segments and multiple copies of the nucleoprotein arranged in a double helical conformation. vRNPs are macromolecular machines responsible for messenger RNA synthesis and genome replication, that is, the formation of progeny vRNPs. Here, we describe the structural basis of the transcription process. The mechanism, which we call the 'processive helical track', is based on the extreme flexibility of the helical part of the vRNP that permits a sliding movement between both antiparallel nucleoprotein-RNA strands, thereby allowing the polymerase to move over the genome while bound to both RNA ends. Accordingly, we demonstrate that blocking this movement leads to inhibition of vRNP transcriptional activity. This mechanism also reveals a critical role of the nucleoprotein in maintaining the double helical structure throughout the copying process to make the RNA template accessible to the polymerase.
Identifiants
pubmed: 32152587
doi: 10.1038/s41564-020-0675-3
pii: 10.1038/s41564-020-0675-3
doi:
Substances chimiques
Nucleoproteins
0
RNA, Viral
0
Ribonucleoproteins
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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