Substrate Specificity of OXA-48 after β5-β6 Loop Replacement.
carbapenemase
expanded-spectrum cephalosporins
oxacillinases
β5−β6 loop
Journal
ACS infectious diseases
ISSN: 2373-8227
Titre abrégé: ACS Infect Dis
Pays: United States
ID NLM: 101654580
Informations de publication
Date de publication:
08 05 2020
08 05 2020
Historique:
pubmed:
12
3
2020
medline:
17
6
2021
entrez:
12
3
2020
Statut:
ppublish
Résumé
OXA-48 carbapenemase has rapidly spread in many countries worldwide with several OXA-48-variants being described, differing by a few amino acid (AA) substitutions or deletions, mostly in the β5-β6 loop. While single AA substitutions have only a minor impact on OXA-48 hydrolytic profiles, others with 4 AA deletions result in loss of carbapenem hydrolysis and gain of expanded-spectrum cephalosporin (ESC) hydrolysis. We have replaced the β5-β6 loop of OXA-48 with that of OXA-18, a clavulanic-acid inhibited oxacillinase capable of hydrolyzing ESCs but not carbapenems. The hybrid enzyme OXA-48Loop18 was able to hydrolyze ESCs and carbapenems (although with a lower
Identifiants
pubmed: 32156115
doi: 10.1021/acsinfecdis.9b00452
doi:
Substances chimiques
Carbapenems
0
Cephalosporins
0
beta-Lactamases
EC 3.5.2.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM