MicroRNA-mRNA networks define translatable molecular outcome phenotypes in osteosarcoma.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
10 03 2020
Historique:
received: 17 11 2019
accepted: 03 02 2020
entrez: 12 3 2020
pubmed: 12 3 2020
medline: 15 12 2020
Statut: epublish

Résumé

There is a lack of well validated prognostic biomarkers in osteosarcoma, a rare, recalcitrant disease for which treatment standards have not changed in over 20 years. We performed microRNA sequencing in 74 frozen osteosarcoma biopsy samples, constituting the largest single center translationally analyzed osteosarcoma cohort to date, and we separately analyzed a multi-omic dataset from a large NCI supported national cooperative group cohort. We validated the prognostic value of candidate microRNA signatures and contextualized them in relevant transcriptomic and epigenomic networks. Our results reveal the existence of molecularly defined phenotypes associated with outcome independent of clinicopathologic features. Through machine learning based integrative pharmacogenomic analysis, the microRNA biomarkers identify novel therapeutics for stratified application in osteosarcoma. The previously unrecognized osteosarcoma subtypes with distinct clinical courses and response to therapy could be translatable for discerning patients appropriate for more intensified, less intensified, or alternate therapeutic regimens.

Identifiants

pubmed: 32157112
doi: 10.1038/s41598-020-61236-3
pii: 10.1038/s41598-020-61236-3
pmc: PMC7064533
doi:

Substances chimiques

Biomarkers, Tumor 0
MicroRNAs 0
RNA, Messenger 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4409

Subventions

Organisme : NCI NIH HHS
ID : R01 CA178908
Pays : United States
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : R35CA220523
Pays : International

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Auteurs

Christopher E Lietz (CE)

Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Cassandra Garbutt (C)

Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.
Illumina, Inc., San Diego, United States.

William T Barry (WT)

Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States.

Vikram Deshpande (V)

Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Yen-Lin Chen (YL)

Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Santiago A Lozano-Calderon (SA)

Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Yaoyu Wang (Y)

Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States.
Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, United States.
Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, United States.

Brian Lawney (B)

Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States.
Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, United States.
Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, United States.

David Ebb (D)

Pediatric Hematology-Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Gregory M Cote (GM)

Department of Hematology/Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Zhenfeng Duan (Z)

Department of Orthopaedic Surgery, UCLA, Los Angeles, CA, United States.

Francis J Hornicek (FJ)

Department of Orthopaedic Surgery, UCLA, Los Angeles, CA, United States.

Edwin Choy (E)

Department of Hematology/Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

G Petur Nielsen (G)

Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Benjamin Haibe-Kains (B)

Department of Medical Biophysics, Princess Margaret Cancer Centre, University of Toronto, Toronto, Canada.

John Quackenbush (J)

Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States.
Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, United States.
Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, United States.

Dimitrios Spentzos (D)

Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States. dspentzos@mgh.harvard.edu.

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Classifications MeSH