Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells.

2-Propanol / pharmacology Biocatalysis Culture Media Electrophoresis / methods Escherichia coli / drug effects Solvents / chemistry
Cofactor leaching Dielectrophoresis analysis Microfluidics UV-Vis absorbance analysis Whole-cell biocatalyst

Journal

Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327

Informations de publication

Date de publication:
Jun 2020
Historique:
received: 31 10 2019
accepted: 23 01 2020
revised: 13 01 2020
pubmed: 12 3 2020
medline: 30 1 2021
entrez: 12 3 2020
Statut: ppublish

Résumé

Whole-cell biocatalysts are versatile tools in (industrial) production processes; though, the effects that impact the efficiency are not fully understood yet. One main factor that affects whole-cell biocatalysts is the surrounding medium, which often consists of organic solvents due to low solubility of substrates in aqueous solutions. It is expected that organic solvents change the biophysical and biochemical properties of the whole-cell biocatalysts, e.g. by permeabilising the cell membrane, and thus analysis of these effects is of high importance. In this work, we present an analysis method to study the impact of organic solvents on whole-cell biocatalysts by means of dielectrophoresis. For instance, we evaluate the changes of the characteristic dielectrophoretic trapping ratio induced by incubation of Escherichia coli, serving as a model system, in an aqueous medium containing isopropyl alcohol. Therefore, we could evaluate the impact on the electric polarisability of the cells. For this purpose, a special microchannel device was designed and Escherichia coli cells were genetically modified to reliably synthesise a green fluorescent protein. We could demonstrate that our method was capable of revealing different responses to small changes in isopropyl alcohol concentration and incubation duration. Complementary spectrophotometric UV-Vis (ultraviolet-visible light) absorbance analysis of released NAD(P)

Identifiants

DOI: 10.1007/s00216-020-02451-9 PMID: 32157360 PMC: PMC7235074
pubmed: 32157360
doi: 10.1007/s00216-020-02451-9
pii: 10.1007/s00216-020-02451-9
pmc: PMC7235074
doi:

Substances chimiques

Culture Media 0
Solvents 0
2-Propanol ND2M416302

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3925-3933

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Auteurs

Miriam S Epping (MS)

Experimental Biophysics and Applied Nanosciences, Department of Physics, Bielefeld University, 33615, Bielefeld, Germany.

Severin Wedde (S)

Industrial Organic Chemistry and Biotechnology, Department of Chemistry, Bielefeld University, 33615, Bielefeld, Germany.
Fermentation Engineering, Department of Technology, Bielefeld University, 33615, Bielefeld, Germany.

Armin Grundmann (A)

Experimental Biophysics and Applied Nanosciences, Department of Physics, Bielefeld University, 33615, Bielefeld, Germany.

Marco Radukic (M)

Experimental Biophysics and Applied Nanosciences, Department of Physics, Bielefeld University, 33615, Bielefeld, Germany.
Cellular and Molecular Biotechnology, Department of Technology, Bielefeld University, 33615, Bielefeld, Germany.

Harald Gröger (H)

Industrial Organic Chemistry and Biotechnology, Department of Chemistry, Bielefeld University, 33615, Bielefeld, Germany.

Anke Hummel (A)

Industrial Organic Chemistry and Biotechnology, Department of Chemistry, Bielefeld University, 33615, Bielefeld, Germany.

Martina Viefhues (M)

Experimental Biophysics and Applied Nanosciences, Department of Physics, Bielefeld University, 33615, Bielefeld, Germany. viefhues@physik.uni-bielefeld.de.

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Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Alcools › Propanols › Propan-2-ol › Dielectrophoretic analysis of the impact of...
questionsmedicales.fr › Métabolisme › Biocatalyse › Dielectrophoretic analysis of the impact of...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Dielectrophoretic analysis of the impact of...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Dielectrophoretic analysis of the impact of...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Dielectrophoretic analysis of the impact of...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Solvants › Dielectrophoretic analysis of the impact of...