Genome sequencing of ion-beam-induced mutants facilitates detection of candidate genes responsible for phenotypes of mutants in rice.
Dwarf
Exome
Heading date
Ion beam
Mutagenesis
Rice
Journal
Mutation research
ISSN: 1873-135X
Titre abrégé: Mutat Res
Pays: Netherlands
ID NLM: 0400763
Informations de publication
Date de publication:
Historique:
received:
24
12
2019
revised:
07
02
2020
accepted:
17
02
2020
pubmed:
15
3
2020
medline:
15
12
2020
entrez:
15
3
2020
Statut:
ppublish
Résumé
Ion beams are physical mutagens used for plant and microbe breeding that cause mutations via a mechanism distinct from those of chemical mutagens or gamma rays. We utilized whole-exome sequencing of rice DNA in order to understand the properties of ion beam-induced mutations in a genome-wide manner. DNA libraries were constructed from selected carbon-ion-beam-induced rice mutants by capturing with a custom probes covering 66.3 M bases of nearly all exons and miRNAs predicted in the genome. A total of 56 mutations, including 24 single nucleotide variations, 23 deletions, and 5 insertions, were detected in five mutant rice lines (two dwarf and three early-heading-date mutants). The mutations were distributed among all 12 chromosomes, and the average mutation frequency in the M1 generation was estimated to be 2.7 × 10
Identifiants
pubmed: 32171089
pii: S0027-5107(19)30129-0
doi: 10.1016/j.mrfmmm.2020.111691
pii:
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111691Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.