A Versatile Toolkit for Controllable and Highly Selective Multifunctionalization of Bacterial Magnetic Nanoparticles.
Magnetospirillum gryphiswaldense
magnetic composites
magnetosomes
nanoparticles
synthetic biology
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
29
11
2019
revised:
21
02
2020
accepted:
24
02
2020
pubmed:
19
3
2020
medline:
22
6
2021
entrez:
19
3
2020
Statut:
ppublish
Résumé
Their unique material characteristics, i.e. high crystallinity, strong magnetization, uniform shape and size, and the ability to engineer the enveloping membrane in vivo make bacterial magnetosomes highly interesting for many biomedical and biotechnological applications. In this study, a versatile toolkit is developed for the multifunctionalization of magnetic nanoparticles in the magnetotactic bacterium Magnetospirillum gryphiswaldense, and the use of several abundant magnetosome membrane proteins as anchors for functional moieties is explored. High-level magnetosome display of cargo proteins enables the generation of engineered nanoparticles with several genetically encoded functionalities, including a core-shell structure, magnetization, two different catalytic activities, fluorescence and the presence of a versatile connector that allows the incorporation into a hydrogel-based matrix by specific coupling reactions. The resulting reusable magnetic composite demonstrates the high potential of synthetic biology for the production of multifunctional nanomaterials, turning the magnetosome surface into a platform for specific versatile display of functional moieties.
Identifiants
pubmed: 32187836
doi: 10.1002/smll.201906922
doi:
Substances chimiques
Bacterial Proteins
0
Magnetite Nanoparticles
0
Membrane Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1906922Informations de copyright
© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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