Synthesis of phosphoramidate-linked DNA by a modified DNA polymerase.
NP-DNA
XNA
alternative genetic polymers
polymerases
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
31 03 2020
31 03 2020
Historique:
pubmed:
20
3
2020
medline:
15
8
2020
entrez:
20
3
2020
Statut:
ppublish
Résumé
All known polymerases copy genetic material by catalyzing phosphodiester bond formation. This highly conserved activity proceeds by a common mechanism, such that incorporated nucleoside analogs terminate chain elongation if the resulting primer strand lacks a terminal hydroxyl group. Even conservatively substituted 3'-amino nucleotides generally act as chain terminators, and no enzymatic pathway for their polymerization has yet been found. Although 3'-amino nucleotides can be chemically coupled to yield stable oligonucleotides containing N3'→P5' phosphoramidate (NP) bonds, no such internucleotide linkages are known to occur in nature. Here, we report that 3'-amino terminated primers are, in fact, slowly extended by the DNA polymerase from
Identifiants
pubmed: 32188786
pii: 1922400117
doi: 10.1073/pnas.1922400117
pmc: PMC7132125
doi:
Substances chimiques
Amides
0
Oligonucleotides
0
Phosphoric Acids
0
RNA
63231-63-0
DNA
9007-49-2
phosphoramidic acid
9Q189608GB
DNA-Directed DNA Polymerase
EC 2.7.7.7
Banques de données
PDB
['6UR2', '6UR4', '6UR9', '6US5']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7276-7283Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
Copyright © 2020 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
Competing interest statement: The authors have filed for a patent related to this work.
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