Molecular dissection of pheromone selectivity in the competence signaling system ComRS of streptococci.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
07 04 2020
Historique:
pubmed: 22 3 2020
medline: 15 7 2020
entrez: 22 3 2020
Statut: ppublish

Résumé

Competence allows bacteria to internalize exogenous DNA fragments for the acquisition of new phenotypes such as antibiotic resistance or virulence traits. In most streptococci, competence is regulated by ComRS signaling, a system based on the mature ComS pheromone (XIP), which is internalized to activate the (R)RNPP-type ComR sensor by triggering dimerization and DNA binding. Cross-talk analyses demonstrated major differences of selectivity between ComRS systems and raised questions concerning the mechanism of pheromone-sensor recognition and coevolution. Here, we decipher the molecular determinants of selectivity of the closely related ComRS systems from

Identifiants

pubmed: 32198205
pii: 1916085117
doi: 10.1073/pnas.1916085117
pmc: PMC7149491
doi:

Substances chimiques

Pheromones 0
Luciferases EC 1.13.12.-

Banques de données

PDB
['6HU8', '6HUA', '6QER']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7745-7754

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Laura Ledesma-Garcia (L)

Louvain Institute of Biomolecular Science and Technology, Biochemistry and Genetics of Microorganisms, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium.

Jordhan Thuillier (J)

Institute of Integrative Biology of the Cell (I2BC), University Paris-Sarclay, CEA, CNRS, 91198, Gif-sur-Yvette, France.

Armando Guzman-Espinola (A)

Louvain Institute of Biomolecular Science and Technology, Biochemistry and Genetics of Microorganisms, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium.

Imke Ensinck (I)

Louvain Institute of Biomolecular Science and Technology, Biochemistry and Genetics of Microorganisms, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium.

Inès Li de la Sierra-Gallay (I)

Institute of Integrative Biology of the Cell (I2BC), University Paris-Sarclay, CEA, CNRS, 91198, Gif-sur-Yvette, France.

Noureddine Lazar (N)

Institute of Integrative Biology of the Cell (I2BC), University Paris-Sarclay, CEA, CNRS, 91198, Gif-sur-Yvette, France.

Magali Aumont-Nicaise (M)

Institute of Integrative Biology of the Cell (I2BC), University Paris-Sarclay, CEA, CNRS, 91198, Gif-sur-Yvette, France.

Johann Mignolet (J)

Louvain Institute of Biomolecular Science and Technology, Biochemistry and Genetics of Microorganisms, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium.

Patrice Soumillion (P)

Louvain Institute of Biomolecular Science and Technology, Biochemistry and Genetics of Microorganisms, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium.

Sylvie Nessler (S)

Institute of Integrative Biology of the Cell (I2BC), University Paris-Sarclay, CEA, CNRS, 91198, Gif-sur-Yvette, France sylvie.nessler@i2bc.paris-saclay.fr pascal.hols@uclouvain.be.

Pascal Hols (P)

Louvain Institute of Biomolecular Science and Technology, Biochemistry and Genetics of Microorganisms, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium; sylvie.nessler@i2bc.paris-saclay.fr pascal.hols@uclouvain.be.

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