Poly I:C stimulation in-vitro as a marker for an antiviral response in different cell types generated from Buffalo (Bubalus bubalis).
Animals
Buffaloes
/ immunology
Cells, Cultured
DEAD Box Protein 58
/ immunology
Disease Resistance
/ immunology
Fibroblasts
/ drug effects
Host-Pathogen Interactions
/ drug effects
Immunity, Innate
/ drug effects
Interferon-beta
/ immunology
Macrophages, Alveolar
/ drug effects
Monocytes
/ drug effects
Poly I-C
/ pharmacology
Primary Cell Culture
Signal Transduction
/ drug effects
Antiviral
Buffalo
Innate
Macrophage
RLR
Journal
Molecular immunology
ISSN: 1872-9142
Titre abrégé: Mol Immunol
Pays: England
ID NLM: 7905289
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
29
08
2019
revised:
06
02
2020
accepted:
09
03
2020
pubmed:
23
3
2020
medline:
14
7
2020
entrez:
23
3
2020
Statut:
ppublish
Résumé
The innate immune system is activated upon virus invasion of a host cell by recognizing viral component, such as dsRNA through specific receptors, resulting in the production of type- I IFNs, which confer an antiviral state within the invaded as well as surrounding cells. In the present study, fibroblast, monocyte and macrophage cells derived from water Buffalo (Bubalus bubalis) were exposed to a synthetic dsRNA analogue, poly I:C to mimic viral invasion in each cell type. Recognition of poly I:C through cytosolic helicase receptors RIG-I and MDA5 molecule lead to the activation of the RLR pathway, subsequently activating the MAVS-IRF3/7 cascade and the production of antiviral effector molecule like IFNβ and ISGs. Within the different cell types, we identified variability in RLR receptor and IFNβ expression after poly I:C administration. Fibroblasts responded quickly and strongly with IFNβ production, followed by macrophages and monocytes. Despite absolute expression variability among different cell types the expression trend of RLRs pathway genes were similar. Length of poly I:C molecule also influence IFNβ expression in response of RLR pathway. Short (LMW) poly I:C induce stronger IFN-β expression in myeloid (macrophage and monocyte) cells. In contrast long (HMW) poly I:C preferably elicit higher IFNβ expression in non-myeloid (fibroblast) cell. Therefore, MDA5 and RIG-1 plays an indispensable role in eliciting antiviral response in non- immune (fibroblast) host cell. Thus, stimulation of RLR pathway with suitable and potentially cell-type specific agonist molecules successfully elicit antiviral state in the host animal, with fibroblasts conferring a stronger antiviral state compared with the monocytes and macrophages.
Identifiants
pubmed: 32200171
pii: S0161-5890(19)30607-8
doi: 10.1016/j.molimm.2020.03.004
pii:
doi:
Substances chimiques
Interferon-beta
77238-31-4
DEAD Box Protein 58
EC 3.6.4.13
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
136-143Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.