Nanofocused Scanning X-ray Fluorescence Microscopy Revealing an Effect of Heterozygous Hemoglobin S and C on Biochemical Activities in

Cells, Cultured Erythrocytes / metabolism Hemoglobin C / analysis Hemoglobin, Sickle / analysis Humans Microscopy, Fluorescence Nanotechnology Plasmodium falciparum / metabolism X-Rays

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
21 04 2020
Historique:
pubmed: 24 3 2020
medline: 11 2 2021
entrez: 24 3 2020
Statut: ppublish

Résumé

While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life-threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spatial distribution of individual elements in subcellular compartments, including Fe, S, P, Zn, and Cu, in

Identifiants

DOI: 10.1021/acs.analchem.9b05111 PMID: 32202408
pubmed: 32202408
doi: 10.1021/acs.analchem.9b05111
doi:

Substances chimiques

Hemoglobin, Sickle 0
Hemoglobin C 9008-00-8

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5765-5771

Auteurs

Benjamin Fröhlich (B)

Physical Chemistry of Biosystems, Institute of Physical Chemistry, Heidelberg University, D-69120 Heidelberg, Germany.

Yang Yang (Y)

The European Synchrotron Radiation Facility (ESRF), 38043 Grenoble, France.
National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York 11973, United States.
ORCID: 0000-0001-5318-5385

Judith Thoma (J)

Physical Chemistry of Biosystems, Institute of Physical Chemistry, Heidelberg University, D-69120 Heidelberg, Germany.

Julian Czajor (J)

Physical Chemistry of Biosystems, Institute of Physical Chemistry, Heidelberg University, D-69120 Heidelberg, Germany.

Christine Lansche (C)

Department of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, D-69120 Heidelberg, Germany.

Cecilia Sanchez (C)

Department of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, D-69120 Heidelberg, Germany.

Michael Lanzer (M)

Department of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, D-69120 Heidelberg, Germany.

Peter Cloetens (P)

The European Synchrotron Radiation Facility (ESRF), 38043 Grenoble, France.
ORCID: 0000-0002-4129-9091

Motomu Tanaka (M)

Physical Chemistry of Biosystems, Institute of Physical Chemistry, Heidelberg University, D-69120 Heidelberg, Germany.
Center for Integrative Medicine and Physics, Institute for Advanced Study, Kyoto University, 606-8501 Kyoto, Japan.
ORCID: 0000-0003-3663-9554

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Érythrocytes › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Globines › Hémoglobines › Hémoglobines anormales › Hémoglobine C › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Globines › Hémoglobines › Hémoglobines anormales › Hémoglobine S › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Miniaturisation › Nanotechnologie › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Haemosporida › Plasmodium › Plasmodium falciparum › Nanofocused Scanning X-ray Fluorescence...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement ionisant › Rayons X › Nanofocused Scanning X-ray Fluorescence...