3D Bioprinting and Differentiation of Primary Skeletal Muscle Progenitor Cells.
Actins
/ analysis
Animals
Bioprinting
/ methods
Cell Encapsulation
Cells, Cultured
Equipment Design
Fluorescent Dyes
Gelatin
Hydrogels
Male
Methacrylates
Mice
Mice, Inbred C57BL
Muscle Development
Muscle Fibers, Skeletal
/ chemistry
Myoblasts
/ chemistry
Printing, Three-Dimensional
Tissue Engineering
/ methods
Tissue Scaffolds
3D bioprinting
Myoblasts
Skeletal muscle
Tissue engineering
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
25
3
2020
pubmed:
25
3
2020
medline:
9
3
2021
Statut:
ppublish
Résumé
Volumetric loss of skeletal muscle can occur through sports injuries, surgical ablation, trauma, motor or industrial accident, and war-related injury. Likewise, massive and ultimately catastrophic muscle cell loss occurs over time with progressive degenerative muscle diseases, such as the muscular dystrophies. Repair of volumetric loss of skeletal muscle requires replacement of large volumes of tissue to restore function. Repair of larger lesions cannot be achieved by injection of stem cells or muscle progenitor cells into the lesion in absence of a supportive scaffold that (1) provides trophic support for the cells and the recipient tissue environment, (2) appropriate differentiational cues, and (3) structural geometry for defining critical organ/tissue components/niches necessary or a functional outcome. 3D bioprinting technologies offer the possibility of printing orientated 3D structures that support skeletal muscle regeneration with provision for appropriately compartmentalized components ranging across regenerative to functional niches. This chapter includes protocols that provide for the generation of robust skeletal muscle cell precursors and methods for their inclusion into methacrylated gelatin (GelMa) constructs using 3D bioprinting.
Identifiants
pubmed: 32207116
doi: 10.1007/978-1-0716-0520-2_15
doi:
Substances chimiques
Actins
0
Fluorescent Dyes
0
Hydrogels
0
Methacrylates
0
Gelatin
9000-70-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
229-242Références
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