Transcription Factor Binding to Replicated DNA.

DNA Replication DNA, Fungal / metabolism Genome, Fungal Protein Binding RNA Polymerase II / metabolism S Phase Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / metabolism Transcription Factors / metabolism
DNA RNA polymerase chromatin replication transcription transcription factor

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
24 03 2020
Historique:
received: 07 10 2019
revised: 13 01 2020
accepted: 27 02 2020
entrez: 27 3 2020
pubmed: 27 3 2020
medline: 10 4 2021
Statut: ppublish

Résumé

Genome replication perturbs the DNA regulatory environment by displacing DNA-bound proteins, replacing nucleosomes, and introducing dosage imbalance between regions replicating at different S-phase stages. Recently, we showed that these effects are integrated to maintain transcription homeostasis: replicated genes increase in dosage, but their expression remains stable due to replication-dependent epigenetic changes that suppress transcription. Here, we examine whether reduced transcription from replicated DNA results from limited accessibility to regulatory factors by measuring the time-resolved binding of RNA polymerase II (Pol II) and specific transcription factors (TFs) to DNA during S phase in budding yeast. We show that the Pol II binding pattern is largely insensitive to DNA dosage, indicating limited binding to replicated DNA. In contrast, binding of three TFs (Reb1, Abf1, and Rap1) to DNA increases with the increasing DNA dosage. We conclude that the replication-specific chromatin environment remains accessible to regulatory factors but suppresses RNA polymerase recruitment.

Identifiants

DOI: 10.1016/j.celrep.2020.02.114 PMID: 32209462
pubmed: 32209462
pii: S2211-1247(20)30295-3
doi: 10.1016/j.celrep.2020.02.114
pii:
doi:

Substances chimiques

DNA, Fungal 0
Saccharomyces cerevisiae Proteins 0
Transcription Factors 0
RNA Polymerase II EC 2.7.7.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3989-3995.e4

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

Auteurs

Raz Bar-Ziv (R)

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel; The Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA 94720, USA.

Sagie Brodsky (S)

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.

Michal Chapal (M)

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.

Naama Barkai (N)

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address: naama.barkai@weizmann.ac.il.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Réplication de l'ADN › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN fongique › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome fongique › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › RNA polymerase II › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Phénomènes génétiques › Réplication de l'ADN › Phase S › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › Transcription Factor Binding to Replicated DNA.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Transcription Factor Binding to Replicated DNA.