A Scalable Purification Method for Mitochondria from Trypanosoma brucei.

Cell Fractionation / instrumentation Centrifugation, Density Gradient / instrumentation Mitochondria Trypanosoma brucei brucei / cytology
Mitochondria Nitrogen cavitation Subcellular fractionation Trypanosoma brucei Trypanosomes

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 30 3 2020
pubmed: 30 3 2020
medline: 2 2 2021
Statut: ppublish

Résumé

Due to its unique biology the mitochondrion of Trypanosoma brucei has attracted a lot of interest since many decades, making it arguably the best studied mitochondrion outside yeast and mammals. Here we describe a method allowing purification of mitochondria from procyclic trypanosomes that yields highly enriched and functional organelles. The method is based on isotonic lysis of cells by nitrogen cavitation, DNase I digestion, differential centrifugation and Nycodenz gradient centrifugation. The method is scalable and can be adapted to culture volumes a small as 100 mL or as large as 24 L.

Identifiants

DOI: 10.1007/978-1-0716-0294-2_36 PMID: 32221945
pubmed: 32221945
doi: 10.1007/978-1-0716-0294-2_36
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

611-626

Auteurs

Moritz Niemann (M)

Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.

André Schneider (A)

Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland. andre.schneider@dcb.unibe.ch.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Fractionnement cellulaire › A Scalable Purification Method for...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Ultracentrifugation › Centrifugation en gradient de densité › A Scalable Purification Method for...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › A Scalable Purification Method for...
questionsmedicales.fr › Eucaryotes › Euglénozoaires › Kinetoplastida › Trypanosomatina › Trypanosoma › Trypanosoma brucei brucei › A Scalable Purification Method for...