Trends and molecular characteristics of carbapenemase-producing Enterobacteriaceae in Japanese hospital from 2006 to 2015.
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
Carbapenem-Resistant Enterobacteriaceae
/ drug effects
Ceftazidime
/ pharmacology
Citrobacter freundii
/ drug effects
DNA, Bacterial
/ genetics
Enterobacteriaceae Infections
/ drug therapy
Gene Transfer, Horizontal
Genes, Bacterial
/ genetics
Hospitals
/ statistics & numerical data
Humans
Imipenem
/ pharmacology
Japan
/ epidemiology
Klebsiella pneumoniae
/ drug effects
Microbial Sensitivity Tests
Multilocus Sequence Typing
Plasmids
/ genetics
Polymerase Chain Reaction
Prevalence
beta-Lactam Resistance
/ genetics
beta-Lactamases
/ genetics
Carbapenemase
Enterobacteriaceae
IncA/C plasmid
IncHI2 plasmid
Plasmid transmission
bla(IMP-1)
Journal
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy
ISSN: 1437-7780
Titre abrégé: J Infect Chemother
Pays: Netherlands
ID NLM: 9608375
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
05
10
2019
revised:
23
01
2020
accepted:
05
02
2020
pubmed:
31
3
2020
medline:
7
4
2021
entrez:
31
3
2020
Statut:
ppublish
Résumé
The increasing number of carbapenemase-producing Enterobacteriaceae (CPE) has become a global problem. Most carbapenemases detected in Japan are imipenemase, which is an imipenem-degrading enzyme with low ability; thus, CPE could have been overlooked. Therefore, this study aimed to detect and analyze CPE, without overlooking CPE showing the low minimum inhibitory concentration phenotype. CPE screening was conducted on 531 ceftazidime-resistant Enterobacteriaceae isolated from Kitasato University Hospital during 2006-2015. We confirmed the presence of the carbapenemase genes (bla The CPE detection rate in Kitasato University Hospital within the past 10 years was 0.0003% (nine CPE strains). These nine CPE strains were identified to harbor 8 bla The CPE detection rate in this study was significantly lower than those previously reported in Japan. In one case, IncA/C plasmid transmission through different bacterial species within the body was speculated. Although the number of CPE detected was low, these results indicated that the resistance plasmid could spread to other bacterial species.
Sections du résumé
BACKGROUND
BACKGROUND
The increasing number of carbapenemase-producing Enterobacteriaceae (CPE) has become a global problem. Most carbapenemases detected in Japan are imipenemase, which is an imipenem-degrading enzyme with low ability; thus, CPE could have been overlooked. Therefore, this study aimed to detect and analyze CPE, without overlooking CPE showing the low minimum inhibitory concentration phenotype.
METHODS
METHODS
CPE screening was conducted on 531 ceftazidime-resistant Enterobacteriaceae isolated from Kitasato University Hospital during 2006-2015. We confirmed the presence of the carbapenemase genes (bla
RESULTS
RESULTS
The CPE detection rate in Kitasato University Hospital within the past 10 years was 0.0003% (nine CPE strains). These nine CPE strains were identified to harbor 8 bla
CONCLUSION
CONCLUSIONS
The CPE detection rate in this study was significantly lower than those previously reported in Japan. In one case, IncA/C plasmid transmission through different bacterial species within the body was speculated. Although the number of CPE detected was low, these results indicated that the resistance plasmid could spread to other bacterial species.
Identifiants
pubmed: 32222331
pii: S1341-321X(20)30043-X
doi: 10.1016/j.jiac.2020.02.002
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
DNA, Bacterial
0
Imipenem
71OTZ9ZE0A
Ceftazidime
9M416Z9QNR
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
667-671Informations de copyright
Copyright © 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None to declare.