Expression of small RNAs of Bordetella pertussis colonizing murine tracheas.
Animals
Bacterial Proteins
/ genetics
Bordetella pertussis
/ genetics
Gene Expression Regulation, Bacterial
/ genetics
Genes, Regulator
/ genetics
Male
Mice
Mice, Inbred C57BL
RNA, Bacterial
/ genetics
RNA, Small Untranslated
/ genetics
Trachea
/ microbiology
Transcription Factors
/ genetics
Virulence
/ genetics
Virulence Factors, Bordetella
/ genetics
Whooping Cough
/ microbiology
Bordetella pertussis
BvgAS
gene regulatory system
in vivo RNA sequencing
small RNA
Journal
Microbiology and immunology
ISSN: 1348-0421
Titre abrégé: Microbiol Immunol
Pays: Australia
ID NLM: 7703966
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
29
01
2020
revised:
22
03
2020
accepted:
24
03
2020
pubmed:
1
4
2020
medline:
23
9
2020
entrez:
1
4
2020
Statut:
ppublish
Résumé
We performed RNA sequencing on Bordetella pertussis, the causative agent of whooping cough, and identified nine novel small RNAs (sRNAs) that were transcribed during the bacterial colonization of murine tracheas. Among them, four sRNAs were more strongly expressed in vivo than in vitro. Moreover, the expression of eight sRNAs was not regulated by the BvgAS two-component system, which is the master regulator for the expression of genes contributing to the bacterial infection. The present results suggest a BvgAS-independent gene regulatory system involving the sRNAs that is active during B. pertussis infection.
Identifiants
pubmed: 32227523
doi: 10.1111/1348-0421.12791
pmc: PMC7317797
doi:
Substances chimiques
Bacterial Proteins
0
RNA, Bacterial
0
RNA, Small Untranslated
0
Transcription Factors
0
Virulence Factors, Bordetella
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
469-475Subventions
Organisme : Japan Society for the Promotion of Science
ID : 17H04075
Organisme : Japan Society for the Promotion of Science
ID : 19K16638
Organisme : Japan Society for the Promotion of Science
ID : 19K23845
Organisme : JSPS KAKENHI
Informations de copyright
© 2020 The Authors. Microbiology and Immunology published by The Societies and John Wiley & Sons Australia, Ltd.
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