Purification and characterization of a novel fenamiphos hydrolysing enzyme from Microbacterium esteraromaticum MM1.
Enolase
Enzyme kinetics
Fenamiphos
Fenamiphos hydrolysing enzyme
Organophosphorus pesticide
Journal
Chemosphere
ISSN: 1879-1298
Titre abrégé: Chemosphere
Pays: England
ID NLM: 0320657
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
11
04
2018
revised:
22
02
2020
accepted:
17
03
2020
pubmed:
2
4
2020
medline:
18
6
2020
entrez:
2
4
2020
Statut:
ppublish
Résumé
Fenamiphos is a neurotoxic organophosphorus pesticide used widely to control pests of crops. Fenamiphos and its toxic oxidation products have been detected in surface and groundwaters. A novel enzyme capable of hydrolysing P-O-C bond of fenamiphos is purified from Microbacterium esteraromaticum MM1 total cellular protein using a combination of methods. The purified fenamiphos hydrolysing enzyme (FHE) was identified as enolase (phosphopyruvate hydratase), a housekeeping enzyme with molecular mass and pI value of 45 kDa and 4.5, respectively. The optimum pH and temperature for the activity of the FHE are 7 and 25 °C, respectively. We studied the influence of metal ions and inhibitors on the enzyme activity. The enzyme was strongly activated by Mg
Identifiants
pubmed: 32229357
pii: S0045-6535(20)30742-6
doi: 10.1016/j.chemosphere.2020.126549
pii:
doi:
Substances chimiques
Organophosphorus Compounds
0
Pesticides
0
fenamiphos
H4NO3L2HBE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126549Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.