Insights into the community structure and lifestyle of the fungal root endophytes of tomato by combining amplicon sequencing and isolation approaches with phytohormone profiling.
amplicon sequencing
fungal endophytes
fungal lifestyle
host genotype
microbiome
phytohormones
Journal
FEMS microbiology ecology
ISSN: 1574-6941
Titre abrégé: FEMS Microbiol Ecol
Pays: England
ID NLM: 8901229
Informations de publication
Date de publication:
01 05 2020
01 05 2020
Historique:
received:
04
12
2019
accepted:
20
03
2020
pubmed:
3
4
2020
medline:
21
11
2020
entrez:
3
4
2020
Statut:
ppublish
Résumé
Little is known about the influence of host genotype and phytohormones on the composition of fungal endophytic communities. We investigated the influence of host genotype and phytohormones on the structure of the fungal endophytic communities of tomato roots by amplicon sequencing of the ITS1 region and combined this approach with isolation and functional characterization of the isolates. A significant effect of the host genotype on the dominant fungal species was found by comparing the cultivars Castlemart and UC82B and, surprisingly, root pathogens were among the most abundant taxa. In contrast, smaller changes in the relative abundance of the dominant species were found in mutants impaired in jasmonic acid biosynthesis (def1) and ethylene biosynthesis (8338) compared to the respective wild types. However, def1 showed significantly higher species richness compared to the wild type. Analysis of the phytohormone profiles of these genotypes indicates that changes in the phytohormone balance may contribute to this difference in species richness. Assessing the lifestyle of isolated fungi on tomato seedlings revealed the presence of both beneficial endophytes and latent pathogens in roots of asymptomatic plants, suggesting that the interactions between members of the microbiome maintain the equilibrium in the community preventing pathogens from causing disease.
Identifiants
pubmed: 32239208
pii: 5815076
doi: 10.1093/femsec/fiaa052
pmc: PMC7174037
pii:
doi:
Substances chimiques
Plant Growth Regulators
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© FEMS 2020.
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