Ethyl methanesulfonate mutant library construction in Gossypium hirsutum L. for allotetraploid functional genomics and germplasm innovation.
Ethyl Methanesulfonate
/ metabolism
Fertility
/ genetics
Gene Library
Genetic Association Studies
Genome, Plant
/ genetics
Genome-Wide Association Study
Germ Cells, Plant
Germination
/ genetics
Gossypium
/ anatomy & histology
Mutagens
/ metabolism
Mutation
/ genetics
Polymorphism, Single Nucleotide
/ genetics
Quantitative Trait, Heritable
Tetraploidy
allotetraploid cotton
ethyl methanesulfonate optimization
functional genomics
genome-wide mutation
germplasm innovation
high-resolution melting
mutant library
phenotypes identification
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
15
09
2019
revised:
13
03
2020
accepted:
16
03
2020
pubmed:
3
4
2020
medline:
6
3
2021
entrez:
3
4
2020
Statut:
ppublish
Résumé
As the gene pool is exposed to both strain on land resources and a lack of diversity in elite allotetraploid cotton, the acquisition and identification of novel alleles has taken on epic importance in facilitating cotton genetic improvement and functional genomics research. Ethyl methanesulfonate (EMS) is an excellent mutagen that induces genome-wide efficient mutations to activate the mutagenic potential of plants with many advantages. The present study established, determined and verified the experimental procedure suitable for EMS-based mutant library construction as the general reference guide in allotetraploid upland cotton. This optimized method and procedure are efficient, and abundant EMS mutant libraries (approximately 12 000) in allotetraploid cotton were successfully obtained. More than 20 mutant phenotypes were observed and screened, including phenotypes of the leaf, flower, fruit, fiber and plant architecture. Through the plants mutant library, high-throughput and high-resolution melting technology-based variation evaluation detected the EMS-induced site mutation. Additionally, based on overall genome-wide mutation analyses by re-sequencing and mutant library assessment, the examination results demonstrated the ideal quality of the cotton EMS-treated mutant library constructed in this study with appropriate high mutation density and saturated genome. What is more, the collection is composed of a broad repertoire of mutants, which is the valuable resource for basic genetic research and functional genomics underlying complex allotetraploid traits, as well as cotton breeding.
Substances chimiques
Mutagens
0
Ethyl Methanesulfonate
9H154DI0UP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
858-868Informations de copyright
© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.
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