Identification of additional /novel QTL associated with resistance to cassava green mite in a biparental mapping population.
Animals
Breeding
/ methods
Chromosome Mapping
/ methods
Chromosomes, Plant
/ genetics
Disease Resistance
/ genetics
Genetic Linkage
/ genetics
Genetic Markers
/ genetics
Male
Manihot
/ genetics
Mites
/ pathogenicity
Nigeria
Phenotype
Plant Diseases
/ genetics
Polymorphism, Single Nucleotide
/ genetics
Quantitative Trait Loci
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
13
11
2019
accepted:
13
03
2020
entrez:
3
4
2020
pubmed:
3
4
2020
medline:
7
7
2020
Statut:
epublish
Résumé
Cassava green mite [CGM, Mononychellus tanajoa (Bondar)] is the most destructive dry-season pest in most cassava production areas. The pest is responsible for cassava fresh root yield losses of over 80%. Deployment of CGM resistant cultivars is the most cost-effective and sustainable approach of alleviating such production losses. The purposes of this study were to validate the stability of CGM resistance genes found in previously published results, to identify new genes for CGM resistance in bi-parental mapping population and estimate the heritability of the trait. A total of 109 F1 progeny derived from a cross between CGM resistant parent, TMEB778 and a very susceptible parent, TMEB419 were evaluated under CGM hotspot areas in Nigeria for two cropping seasons. A total of 42,204 SNP markers with MAF ≥ 0.05 were used for single-marker analysis. The most significant QTL (S12_7962234) was identified on the left arm on chromosome 12 which explained high phenotypic variance and harboured significant single nucleotide polymorphism (SNP) markers conferring resistance to CGM and leaf pubescence (LP). Colocalization of the most significant SNP associated with resistance to CGM and LP on chromosome 12 is possibly an indication of a beneficial pleiotropic effect or are physically linked. These significant SNPs markers were intersected with the gene annotations and 33 unique genes were identified within SNPs at 4 - 8MB on chromosome 12. Among these genes, nine novel candidate genes namely; Manes.12077600, Manes.12G086200, Manes.12G061200, Manes.12G083100, Manes.12G082000, Manes.12G094100, Manes.12G075600, Manes.12G091400 and Manes.12G069300 highly expressed direct link to cassava green mite resistance. Pyramiding the new QTL/genes identified on chromosome 12 in this study with previously discovered loci, such on chromosome 8, will facilitate breeding varieties that are highly resistant CGM.
Identifiants
pubmed: 32240258
doi: 10.1371/journal.pone.0231008
pii: PONE-D-19-29422
pmc: PMC7117712
doi:
Substances chimiques
Genetic Markers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0231008Déclaration de conflit d'intérêts
The authors have declared no competing interests exist.
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