Rhamnolipids from Planococcus spp. and their mechanism of action against pathogenic bacteria.
Bactericidal
Biosurfactant
Planococcus sp.
Rhamnolipid
Vegetable oil
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
31
01
2020
revised:
12
03
2020
accepted:
14
03
2020
pubmed:
3
4
2020
medline:
17
4
2020
entrez:
3
4
2020
Statut:
ppublish
Résumé
Two bacterial species with the ability to produce biosurfactants were isolated from a pesticide contaminated soil and identified as Planococcus rifietoensis IITR53 and Planococcus halotolerans IITR55. Formation of froth indicating the surfactant production was observed when grown in basal salt medium containing 2% glucose. The culture supernatant after 72 h showed reduction in surface tension from 72 N/m to 46 and 42 N/m for strain IITR53 and IITR55 with emulsification index of 51 and 54% respectively. The biosurfactant identified as rhamnolipid based on liquid chromatography-mass spectrometry analysis, was found to inhibit the growth of both gram- positive and negative pathogenic bacteria. Both the rhamnolipids at 40 mg/mL exhibited the release of extracellular DNA and protein content. Also at one third of the MIC, a significant generation of reactive oxygen species was recorded. These rhamnolipids effectively emulsified different vegetable oils suggesting their possible utilization as antimicrobial agent.
Identifiants
pubmed: 32240926
pii: S0960-8524(20)30477-6
doi: 10.1016/j.biortech.2020.123206
pii:
doi:
Substances chimiques
Glycolipids
0
Surface-Active Agents
0
rhamnolipid
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
123206Informations de copyright
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