Expression analysis of taste receptor genes (T1R1, T1R3, and T2R4) in response to bacterial, viral and parasitic infection in rainbow trout, Oncorhynchus mykiss.
Amino Acid Sequence
Animals
Ciliophora Infections
/ immunology
Fish Diseases
/ immunology
Fish Proteins
/ genetics
Flavobacteriaceae Infections
/ immunology
Flavobacterium
/ physiology
Gene Expression Profiling
/ veterinary
Gene Expression Regulation
/ immunology
Hymenostomatida
/ physiology
Immunity, Innate
/ genetics
Immunity, Mucosal
/ genetics
Infectious hematopoietic necrosis virus
/ physiology
Oncorhynchus mykiss
/ genetics
Phylogeny
Receptors, G-Protein-Coupled
/ genetics
Rhabdoviridae Infections
/ immunology
Sequence Alignment
/ veterinary
Flavobacterium cloumnare
IHNV
Ichthyophthirius multifiliis
Oncorhynchus mykiss
Taste receptor
Journal
Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
29
01
2020
revised:
22
03
2020
accepted:
25
03
2020
pubmed:
4
4
2020
medline:
13
1
2021
entrez:
4
4
2020
Statut:
ppublish
Résumé
Emerging evidence suggests that bitter and sweet Taste receptors (TRs) in the airway are important sentinels of innate immunity. TRs are G protein-coupled receptors that trigger downstream signaling cascades in response to activation of specific ligands. Among them, the T1R family consists of three genes: T1R1, T1R2, and T1R3, which function as heterodimers for sweet tastants and umami tastants. While the other TRs family components T2Rs function as bitter tastants. To understand the relationship between TRs and mucosal immunity in teleost, here, we firstly identified and analyzed the molecular characteristics of three TRs (T1R1, T1R3, and T2R4) in rainbow trout (Oncorhynchus mykiss). Secondly, by quantitative real-time PCR (qPCR), we detected the mRNA expression levels of T1R1, T1R3 and T2R4 and found that the three genes could be tested in all detected tissues (pharynx, buccal cavity, tongue, nose, gill, eye, gut, fin, skin) and the expression levels of T1R3 and T2R4 were higher in buccal mucosa (BM) and pharyngeal mucosa (PM) compare to other tissues. It may suggest that T1R3 and T2R4 play important roles in BM and PM. Then, to analyses the changes of expression levels of the three genes in rainbow trout infected with pathogens, we established three infection models Flavobacterium columnare (F. cloumnare), infectious hematopoietic necrosis virus (IHNV) and Ichthyophthirius multifiliis (Ich). Subsequently, by qPCR, we detected the expression profiles of TRs in the gustatory tissues (BM, PM and skin) of rainbow trout after infection with F. cloumnare, IHNV, and Ich, respectively. We found that under three different infection models, the expression of the T1R1, T1R3 and T2R4 showed their own changes in mRNA levels. And the expression levels of the T1R1, T1R3 and T2R4 changed significantly at different time points in response to three infection models, respectively, suggesting that TRs may be associated with mucosal immunity.
Identifiants
pubmed: 32244029
pii: S1050-4648(20)30223-0
doi: 10.1016/j.fsi.2020.03.055
pii:
doi:
Substances chimiques
Fish Proteins
0
Receptors, G-Protein-Coupled
0
taste receptors, type 1
0
taste receptors, type 2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
176-185Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.