A Peptidomimetic Fluorescent Probe to Detect the Trypsin β2 Subunit of the Human 20S Proteasome.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
31 Mar 2020
Historique:
received: 02 03 2020
revised: 25 03 2020
accepted: 29 03 2020
entrez: 5 4 2020
pubmed: 5 4 2020
medline: 5 1 2021
Statut: epublish

Résumé

This work describes the chemical synthesis, combinatorial selection, and enzymatic evaluation of peptidomimetic fluorescent substrates specific for the trypsin-like (β2) subunit of the 20S human proteasome. After deconvolution of a library comprising nearly 6000 compounds composed of peg substituted diaminopropionic acid DAPEG building blocks, the sequence ABZ-Dap(O2(Cbz))-Dap(GO1)-Dap(O2(Cbz))-Arg-ANB-NH

Identifiants

pubmed: 32244300
pii: ijms21072396
doi: 10.3390/ijms21072396
pmc: PMC7177456
pii:
doi:

Substances chimiques

Fluorescent Dyes 0
NCOR1 protein, human 0
Nuclear Receptor Co-Repressor 1 0
Peptidomimetics 0
ortho-Aminobenzoates 0
anthranilic acid 0YS975XI6W
Trypsin EC 3.4.21.4
Proteasome Endopeptidase Complex EC 3.4.25.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Narodowe Centrum Nauki
ID : UMO-2017/27/B/ST5/02061

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Auteurs

Magdalena Wysocka (M)

Faculty of Chemistry, University of Gdansk, Ul. Wita Stwosza 63, 80-308 Gdansk, Poland.

Anita Romanowska (A)

Faculty of Chemistry, University of Gdansk, Ul. Wita Stwosza 63, 80-308 Gdansk, Poland.

Natalia Gruba (N)

Faculty of Chemistry, University of Gdansk, Ul. Wita Stwosza 63, 80-308 Gdansk, Poland.

Michalina Michalska (M)

Faculty of Chemistry, University of Gdansk, Ul. Wita Stwosza 63, 80-308 Gdansk, Poland.

Artur Giełdoń (A)

Faculty of Chemistry, University of Gdansk, Ul. Wita Stwosza 63, 80-308 Gdansk, Poland.

Adam Lesner (A)

Faculty of Chemistry, University of Gdansk, Ul. Wita Stwosza 63, 80-308 Gdansk, Poland.

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Classifications MeSH