Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy-to-Prepare, Low-Cost Zwitterionic Polymer.
Biofunctionalization
Carboxybetaine
Copper-free click chemistry
DNA labeling
Nanoparticle
Poly(isobutylene-alt-maleic anhydride)—PIMA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
5
4
2020
pubmed:
5
4
2020
medline:
9
3
2021
Statut:
ppublish
Résumé
Small, stable, and bright quantum dots (QDs) are of interest in many biosensing and biomedical imaging applications, but current methodologies for obtaining these characteristics can be highly specialized or expensive. We describe a straightforward, low-cost protocol for functionalizing poly(isobutylene-alt-maleic anhydride) (PIMA) with moieties that anchor to the QD surface (histamine), impart hydrophilicity [(2-aminoethyl)trimethylammonium chloride (Me
Identifiants
pubmed: 32246332
doi: 10.1007/978-1-0716-0463-2_7
doi:
Substances chimiques
Cyclooctanes
0
Ligands
0
Maleic Anhydrides
0
Polymers
0
poly(isobutylene-alt-maleic anhydride)
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM