Micro-stepping extended focus reduces photobleaching and preserves structured illumination super-resolution features.

Fluorescent Dyes Lighting Microscopy, Confocal Photobleaching
Extended focus Long-term live-cell imaging Microscopy Photobleaching Rapid projections Super-resolution Well-defined sectioning

Journal

Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457

Informations de publication

Date de publication:
27 05 2020
Historique:
received: 18 10 2019
accepted: 24 03 2020
pubmed: 9 4 2020
medline: 22 6 2021
entrez: 9 4 2020
Statut: epublish

Résumé

Despite progress made in confocal microscopy, even fast systems still have insufficient temporal resolution for detailed live-cell volume imaging, such as tracking rapid movement of membrane vesicles in three-dimensional space. Depending on the shortfall, this may result in undersampling and/or motion artifacts that ultimately limit the quality of the imaging data. By sacrificing detailed information in the

Identifiants

DOI: 10.1242/jcs.240796 PMID: 32265275
pubmed: 32265275
pii: jcs.240796
doi: 10.1242/jcs.240796
pii:
doi:

Substances chimiques

Fluorescent Dyes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© 2020. Published by The Company of Biologists Ltd.

Déclaration de conflit d'intérêts

Competing interestsThe authors have filed a patent with Inven2 (DOFI 19087) and Dehns (ref. 12.147289) regarding the hardware implementation.

Auteurs

Xian Hu (X)

Department of Biosciences, University of Oslo, Blindern, 0371 Oslo, Norway.
ORCID: 0000-0002-3381-7514

Salma Jalal (S)

Mechanobiology Institute, National University of Singapore, Singapore 117411.

Michael Sheetz (M)

Mechanobiology Institute, National University of Singapore, Singapore 117411.
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.
ORCID: 0000-0001-8747-3430

Oddmund Bakke (O)

Department of Biosciences, University of Oslo, Blindern, 0371 Oslo, Norway oddmund.bakke@ibv.uio.no mbifmm@nus.edu.sg.
ORCID: 0000-0003-4843-7626

Felix Margadant (F)

Mechanobiology Institute, National University of Singapore, Singapore 117411 oddmund.bakke@ibv.uio.no mbifmm@nus.edu.sg.
Department of Biomedical Engineering, National University of Singapore, Singapore 117583.
ORCID: 0000-0002-6729-6155

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Micro-stepping extended focus reduces...
questionsmedicales.fr › Environnement et santé publique › Environnement › Environnement contrôlé › Éclairage › Micro-stepping extended focus reduces...
questionsmedicales.fr › Techniques d'investigation › Microscopie confocale › Micro-stepping extended focus reduces...
questionsmedicales.fr › Phénomènes chimiques › Processus photochimiques › Photoblanchiment › Micro-stepping extended focus reduces...