MicroRNA profiling of mouse cortical progenitors and neurons reveals miR-486-5p as a regulator of neurogenesis.
Animals
Blotting, Northern
Computational Biology
/ methods
Electroporation
Female
Gene Expression Profiling
/ methods
High-Throughput Nucleotide Sequencing
/ methods
Immunohistochemistry
In Situ Hybridization
Mice
Mice, Inbred C57BL
MicroRNAs
/ genetics
Neural Stem Cells
/ cytology
Neurogenesis
/ genetics
Neurons
/ cytology
Cortical development
Mouse
Neurogenesis
miR-486a
miR-486b
miRNA
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
11 05 2020
11 05 2020
Historique:
received:
11
03
2020
accepted:
26
03
2020
pubmed:
11
4
2020
medline:
17
12
2020
entrez:
11
4
2020
Statut:
epublish
Résumé
MicroRNAs (miRNAs) are short (∼22 nt) single-stranded non-coding RNAs that regulate gene expression at the post-transcriptional level. Over recent years, many studies have extensively characterized the involvement of miRNA-mediated regulation in neurogenesis and brain development. However, a comprehensive catalog of cortical miRNAs expressed in a cell-specific manner in progenitor types of the developing mammalian cortex is still missing. Overcoming this limitation, here we exploited a double reporter mouse line previously validated by our group to allow the identification of the transcriptional signature of neurogenic commitment and provide the field with the complete atlas of miRNA expression in proliferating neural stem cells, neurogenic progenitors and newborn neurons during corticogenesis. By extending the currently known list of miRNAs expressed in the mouse brain by over twofold, our study highlights the power of cell type-specific analyses for the detection of transcripts that would otherwise be diluted out when studying bulk tissues. We further exploited our data by predicting putative miRNAs and validated the power of our approach by providing evidence for the involvement of miR-486 in brain development.
Identifiants
pubmed: 32273274
pii: dev.190520
doi: 10.1242/dev.190520
pii:
doi:
Substances chimiques
MIRN486 microRNA, mouse
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.